光致电化学鸟嘌呤传感器的制备及其应用  

Preparation and Application of Photoelectrochemical Guanine Sensor

作　　者：赵常志[1] 赵改爽[1] 张兆霞[1] 梁俊钰[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《化学学报》2012年第12期1401-1406,共6页Acta Chimica Sinica

基　　金：山东省自然科学基金(No.2009ZRB01393);电分析化学国家重点实验室开放基金(No.SKLEAC201106)资助项目~~

摘　　要：将硫堇电聚合在光透电极的表面,再利用壳聚糖将黄嘌呤氧化酶固定在具有光电活性的聚硫堇光透电极的表面上制备了光致电化学鸟嘌呤传感器.基于同时具有光敏和电子受体功能的聚硫堇光电界面,该传感器能与黄嘌呤氧化酶催化鸟嘌呤氧化而产生的电子供体(过氧化氢)产生光致电化学响应,通过测量光致电化学反应产生的光电流实现了对鸟嘌呤的检测.文中探讨了传感器的光致电化学响应机理,讨论了偏压、酶量、电解质溶液pH对传感器测定鸟嘌呤的影响.在优化的实验条件下,该传感器对鸟嘌呤的测定范围为1.00～200μmol/L,检出限为0.55μmol/L,9次测定的相对标准偏差小于3.92%.应用该传感器对酸解DNA脱出的鸟嘌呤基和药品阿昔洛韦进行的检测实验显示,相对标准偏差小于5.37%,加标回收率为96.8%～106%.该传感器的制备和对鸟嘌呤的检测不需要过氧化物酶,不需要除氧,有经济、简便等优点.We report herein a new guanine sensor for the determination of guanine based on a photoelec- trochemical effect of poly（thionine） photoelectrode to hydrogen peroxide （H202）. The photoelectrode was fabricated by electropolymerizing thionine on the surface of ITO electrode. And then xanthine oxidase was immobilized on the photoelectrode via the aid of chitosan enwrapping, forming an enzyme-modified photo- electrode. The photoelectrode was employed as an electron acceptor, and H202 from the catalytic reaction of enzyme was employed as an electron donor, the guanine can be detected due to photoelectrochemical effect between photoelectrode and H202. In the paper, photoelectrochemical effects of photoelectrode to H202 and guanine were studied. Effects of bias voltage, illumination intensity and electrolyte pH on the photocurrent were investigated. The linear response of guanine concentrations ranged from 1.00-200 μmol/L was ob- tained with a detection limit of 0.55 μmol/L. The guanine sensor was successfully applied to detect the disorganization of DNA mediated by heating acid solution and active ingredient of aciclovir. The relative standard deviation （RSD） was less than 5.37% and the recovery was in the range of 96.8%- 106% for the determination of samples. The preparation of the sensor and the detection of guanine have the advantages of economy and simpleness due to without peroxidase and deoxygenated.

关 键 词：鸟嘌呤传感器 光致电化学 鸟嘌呤 黄嘌呤氧化酶 鲱鱼精DNA 阿昔洛韦 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]