L-半胱氨酸对农杆菌介导的大豆子叶节转化的影响  

Effects of L-cysteine on Agrobacterium-Mediated Transformation of Soybean Cotyledonary Nodes

作　　者：张福丽[1] 张怡[1] 周玲[1] 谭光轩[1] 陈龙[1] 苑保军 豆颖丰[1] 鲁四海[1] 李成伟[1] 

机构地区：[1]周口师范学院,周口466000 [2]周口市农业科学院

出　　处：《东北林业大学学报》2012年第7期139-141,144,共4页Journal of Northeast Forestry University

基　　金：转基因生物新品种培育重大专项子任务(2008ZX08010-004);国家转基因重大专项(2008ZX08004-001);河南省教育厅自然科学项目(12B210028);周口师范学院实验室开放项目基金;周口师范学院博士科研启动经费支助

摘　　要：通过在共培养基中添加L-半胱氨酸,研究了L-半胱氨酸对农杆菌介导的大豆子叶节转化的影响。结果表明:L-半胱氨酸对大豆子叶节的转化有明显的促进作用,其中质量浓度为500 mg/L时转化效率最为显著。以此浓度为基础,通过GUS染色和分子检测,得到了大豆转基因植株,使转化效率由不加L-半胱氨酸的1.17%提高到了2.21%。An experiment was conducted to study the effect of L-cysteine on Agrobacterium-mediated genetic transformation of soybean cotyledonary node by adding L-cysteine to co-culture medium. Result showed that L-cysteine could obviously pro- mote the transformation efficiency of soybean cotyledonary node. The most significant effect was observed when the concentration of L-cysteine was 500 mg/L. On the base of the optimum concentration, transgenic plants of soybean were generated and detected by GUS staining and molecular detection. The transformation rate in the absence of L-cysteine was 1.17%, but it was improved to 2.21% in the presence of 500 mg/L L-cysteine in co-culture medium.

关 键 词：:L-半胱氨酸 农杆菌介导 大豆子叶节 转化 

分 类 号：Q785[生物学—分子生物学]