脂质体介导的VEGF质粒对成年大鼠脑缺血再灌注后大脑皮质内P-MeCP2表达的影响  

Influence of liposome-mediated VEGF plasmid on the expression of P-MeCP2 in cortex of the adult rats after brain ischemia and reperfusion injury

作　　者：倪晶晶[1] 王俊波[2] 孟香红[1] 任典寰[1] 陶冬英[1] 

机构地区：[1]宁波天一职业技术学院人体形态学教研室,浙江宁波315104 [2]浙江大学城市学院医学与生命科学学院,浙江杭州310015

出　　处：《解剖科学进展》2012年第4期352-356,共5页Progress of Anatomical Sciences

基　　金：浙江省教育厅高校科研计划项目(No.Y201122611)

摘　　要：目的观察脂质体介导的VEGF质粒对成年大鼠脑缺血再灌注后大脑神经元phosphorylation of methyl-CpG binding protein 2 (P-MeCP2)表达的影响,探讨VEGF促进缺血损伤后神经元新生的可能机制,进一步为VEGF治疗缺血性脑中风提供实验和理论依据。方法 30只SD大鼠,随机分为假手术组(sham组)、质粒组(VEGF组)和对照质粒组(vehicle组),采用左侧大脑中动脉线栓(MCAO)模型,侧脑室给药,免疫印迹、免疫荧光三标染色及激光共聚焦扫描技术等方法检测P-MeCP2的表达。结果再灌2周VEGF质粒组P-MeCP2、BrdU和NeuN同时表达于缺血侧大脑皮质神经元,而缺血对侧没有找到,VEGF质粒组皮质内P-MeCP2的表达较对照组、假手术组显著增高(p<0.01)。结论脂质体介导的VEGF质粒能促进成年大鼠脑缺血神经元的新生,可能与上调P-MeCP2的表达有关。Objective To observe the influence of liposome-mediated VEGF plasmid on the expression of P- MeCP2 in cortex of the adult rats after brain ischemia and reperfusion injury. Methods Thirty Sprague-Dawley rats were assigned randomly into a VEGF group（experimental group）, a sham group（placebo group） and a vehicle group （control group）. Middle cerebral artery occlusion（MCAO） was applied as cerebral ischemia model. Liposome-mediated VEGF plasmid was injected into lateral ventricle, western blotting, triple immunofluorescence staining and confocal laser scanning techniques were utilized to examine the expression of P-MeCP2. Results Co-localizing of P-MeCP2 with BrdU and NeuN was found in cerebral cortex neurons of ischemic hemisphere, but not found in the opposite side in 2w VEGF group. The expression level of P-MeCP2 was significantly higher in 2w VEGF group than in sham control group and the placebo group （p〈0.01 ） . Conclusion Neogenesis of liposome-mediated VEGF plasmid might be related to upregulation of P-MeCP2 expression in brain ischemia and reperfusion injury rats.

关 键 词：脑缺血再灌注损伤 P-MeCP2 血管内皮生长因子 免疫印迹 免疫荧光 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]