细胞因子抗体芯片在筛选NEC血清蛋白标记物中的应用价值  

Value of application of cytokine antibody chip for screening of serum protein biomarker of patients with NEC

作　　者：张磊[1] 秦克旺[1] 

机构地区：[1]广州医学院附属广东省妇儿医院外科,广东广州510010

出　　处：《中国妇幼保健》2012年第19期3036-3040,共5页Maternal and Child Health Care of China

摘　　要：目的:应用细胞因子抗体芯片(cytokine antibodychip,CAC)分析新生儿坏死性小肠结肠炎(neonatal necrotiz-ing enterocolitis,NEC)患儿血清中炎性细胞因子表达的变化。方法:采用CAC技术平台测定6例NEC患儿(NEC组)和4例正常健康人(对照组)血清中79种细胞因子。按照至少在4个样本中信号值>400为表达有意义的细胞因子,筛选出有表达意义的细胞因子,应用芯片差异显著性分析(significance analysis of microarray,SAM)软件对有表达意义的细胞因子进行差异表达分析,初步获得NEC差异表达细胞因子。结果:筛选到白介素-8(IL-8),表皮生长因子(EGF),巨噬细胞炎性蛋白-1β(MIP-1β)和生长相关致癌基因(GRO)等4个在NEC患儿血清中低表达的细胞因子以及瘦素(Leptin)这一高表达细胞因子。聚类分析表明,上述5个差异表达的细胞因子能够明显区分NEC组和正常健康对照组。结论:CAC是一种高效、快速的蛋白质组学技术,利用CAC从血清中筛选NEC相关蛋白分子标记物是可行的。Objective：To analyze the changes of expressions of inflammatory cytokines in serum of children with neonatal necrotizing enterocolitis（NEC） by cytokine antibody chip.Methods：CAC technology platform was used to detect 79 kinds of cytokines in serum samples of 6 children with NEC（NEC group） and 4 normal children（control group）.The differential expression cytokines were screened out according to signal value〉400 among at least 4 samples;significance analysis of microarray（SAM） was used for differential expression analysis of the cytokines,NEC differential expression cytokines were obtained preliminarily.Results：Four kinds of low expression cytokines in serum（including interleukin-8（IL-8）,epidermal growth factor（EGF）,macrophage inflammatory protein-1β（MIP-1β）,and growth-regulated oncogene（GRO）） and leptin（high expression cytokine） were screened out.Cluster analysis showed that the above-mentioned five cytokines could differentiate NEC group from control group obviously.Conclusion：CAC is an effective and rapid proteomic technology,it is feasible to screen NEC related protein biomarkers from serum by CAC.

关 键 词：新生儿 坏死性小肠结肠炎 细胞因子抗体 芯片 

分 类 号：R574.5[医药卫生—消化系统]