国内6种市售酸奶中的ACE抑制肽研究  

Research on ACE-inhibitory peptide in six domestic commercial yoghurt

作　　者：崔磊[1] 张少辉[1,2] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海交通大学陆伯勋食品安全研究中心,上海200240

出　　处：《中国乳品工业》2012年第6期30-32,42,共4页China Dairy Industry

基　　金：上海市科委2008第14667号(08395820300)项目

摘　　要：选取了不同品牌的6种市售酸奶,通过离心、超滤、凝胶层析分离纯化低分子量(≤1.4 ku)小肽,分析了各酸奶样品中小肽质量浓度及所占比例,并通过ACE抑制实验测定了各小肽收集样的体外抗高血压活性。结果表明,不同市售酸奶的评价指标结果间存在显著性差异(P<0.05),其ACE抑制率依次为样品5(73.0%±2.59%)>样品2(67.3%±3.05%)>样品6(64.2%±3.75%)>样品3(61.9%±2.65%)>样品1(61.7%±2.38%)>样品4(52.4%±3.15%),综合评价样品5为最优。Six commercial yoghurt were chosen in this study. Micropeptides with MW ≤ 1.4 ku were separated and purified by centrifugation, microfiltration and gel fractionation. The content and proportion of micropeptides were analyzed, and the ACE-inhibitory activity of nil- cropeptide fractions was determined. The study showed that the evaluation results of six yoghurt samples were significantly different （P〈0.05）, and the ACE-inhibitory activity was sample 5 （73.0%±2.59%）〉 sample 2 （67.3%±3.05%）〉 sample 6 （64.2%±3.75%）〉 sample 3 （61.9%± 2.65%）〉 sample 1 （61.7%±2.38%）〉 sample 4 （52.4%±3.15%）. The comprehensive characters of sample 5 were the best.

关 键 词：市售酸奶 小肽 抑制ACE活性 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]