光学表面等离子共振生物传感检测系统检测雌二醇的研究  

Study on an optical surface plasmon resonance biosensor system for detecting estradiol in environmental estrogens

作　　者：魏文松[1] 穆琳瑛[1] 李会芹[1] 王顺 李宏伟 侯金博[1] 江敏[3] 胡建东[1] 

机构地区：[1]河南农业大学机电工程学院,河南郑州450002 [2]河南农大迅捷测试技术有限公司,河南郑州450002 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2012年第3期293-297,共5页Journal of Henan Agricultural University

基　　金：国家863计划项目(2007AA100606);河南省杰出青年基金项目(104100510017);河南省教育厅创新人才项目(2009HASTIT019);郑州市科技领军人才项目(096SYJH25101)

摘　　要：设计了一种基于光学表面等离子共振(Surface plasmon resonance,SPR)生物传感检测系统,该生物传感检测系统由集成光学SPR生物传感器、微流池、便捷更换芯片夹具、触摸屏、信号处理电路和USB接口板组成,将该系统用于检测环境刺激中的雌二醇.先在集成光学SPR生物传感器芯片的金膜表面固定雌二醇抗原,然后分别将质量浓度为25,50,100,200 mg.L-1的雌二醇抗原添加到标准质量浓度的样品中进行预反应,将反应后的溶液流过光学SPR生物传感器芯片表面,建立了雌二醇测定标准曲线,其相关系数为0.997 8.An optical surface plasmon resonance （SPR） biosensor detection system was designed, which was used to detect estradiol in environmental estrogens and composed of an integrated optical SPR bio- sensor, a micro-flow cell, a clamp, a touch monitor screen and an electrical control unit （ECU） incor- porated with a photoelectric conversion board and a USB interface board. In this study, the antigen-es- tradiol was immobilized to the surface of the biosensor chip, then the antigen-estradiol samples at 25, 50, 100 , 200 mg ~ L-~ were respectively added into the standard solution of antibody-estradiol for pre- reaction, and the mixture solution after pre-reaction flowed through the surface of the SPR biosensor chip, obtaining the standard curve for detecting estradiol, and coefficient of correlation is 0. 997 8.

关 键 词：光学表面等离子共振 生物传感器 环境雌激素 雌二醇 

分 类 号：S24[农业科学—农业电气化与自动化]