S-acylation-dependent association of the calcium sensor CBL2 with the vacuolar membrane is essential for proper abscisic acid responses  

作　　者：Oliver Batistic Marion Rehers Amir Akerman Kathrin Schlucking Leonie Steinhorst Shaul Yalovsky Jorg Kudla 

机构地区：[1]Institut fiir Biologie und Biotechnologie der Pflanzen, Universit(it Miinster, Schlossplatz 4, Miinster 48149, Germany [2]Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel

出　　处：《Cell Research》2012年第7期1155-1168,共14页细胞研究（英文版）

基　　金：Acknowledgments We thank the ABRC for providing the SALK cbl2-1 T-DNA insertion line. We thank Drs Efraim Lewinsohn and Einat Bar for access to GC/MS. This work was supported by grants from the Israel Science Foundation （ISF 312/07） and the US-ISRAEL Binational Science Foundation （BSF 2009309） to SY and from the Deutsche Forschungsgemeinschaft （SFB629） to JK.

摘　　要：Calcineurin B-like （CBL） proteins contribute to decoding calcium signals by interacting with CBL-interacting protein kinases （CIPKs）. Currently, there is still very little information about the function and specific targeting mechanisms of CBL proteins that are localized at the vacuolar membrane. In this study, we focus on CBL2, an abundant vacuolar membrane-localized calcium sensor of unknown function from Arabidopsis thaliana. We show that vacuolar targeting of CBL2 is specifically brought about by S-acylation of three cysteine residues in its N-terminus and that CBL2 S-acylation and targeting occur by a Brefeldin A-insensitive pathway. Loss of CBL2 function renders plants hypersensitive to the phytohormone abscisic acid （ABA） during seed germination and only fully S-acylated and properly vacuolar-targeted CBL2 proteins can complement this mutant phenotype. These findings define an S-acylation-dependent vacuolar membrane targeting pathway for proteins and uncover a crucial role of vacuolar calcium sensors in ABA responses.

关 键 词：S-ACYLATION vacuolar membrane ABA CBL CALCIUM 

分 类 号：Q257[生物学—细胞生物学] S511.01[农业科学—作物学]