LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration  

作　　者：Mingzhu Fan Chongyi Xu Ke Xu Yuxin Hu 

机构地区：[1]Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China [2]National Center for Plant Gene Research, Beijing 100101, China

出　　处：《Cell Research》2012年第7期1169-1180,共12页细胞研究（英文版）

基　　金：Acknowledgments We are grateful to ABRC and Drs Ben Scheres （UtrechtUniversity, Netherlands）, Jim Haseloff （University of Cambridge, UK）, Nam-Hai Chua （Rockefeller University, USA） for providing the seeds and constructs used in this study. This work was supported by the Ministry of Science and Technology of China （2007CB948200） and by the National Natural Science Foundation of China （30900108 and 30821007）.

摘　　要：The remarkable regeneration capability of plant tissues or organs under culture conditions has underlain an extensive practice for decades. The initial step in plant in vitro regeneration often involves the induction of a pluripotent cell mass termed callus, which is driven by the phytohormone auxin and occurs via a root development pathway. However, the key molecules governing callus formation remain unknown. Here we demonstrate that Arabidopsis LATERAL ORGAN BOUNDARIES DOMAIN （LBD）/ASYMMETRIC LEAVES2-LIKE （ASL） transcription factors are involved in the control of callus formation program. The four LBD genes downstream of AUXIN RESPONSE FACTORs （ARFs）, LBD16, LBD17, LBD18 and LBD29, are rapidly and dramatically induced by callus-inducing medium （CIM） in multiple organs. Ectopic expression of each of the four LBD genes in Arabidopsis is sufficient to trigger spontaneous callus formation without exogenous phytohormones, whereas suppression of LBD function inhibits the callus formation induced by CIM. Moreover, the callus triggered by LBD resembles that induced by CIM by characteristics of ectopically activated root meristem genes and efficient regeneration capacity. These findings define LBD transcription factors as key regulators in the callus induction process, thereby establishing a molecular link between auxin signaling and the plant regeneration program.

关 键 词：LBD callus formation AUXIN REGENERATION ARABIDOPSIS 

分 类 号：S667.903[农业科学—果树学] Q753[农业科学—园艺学]