肾细胞癌组织中TOP2α mRNA、蛋白表达变化及意义  

Expression and clinical significance of TOP2α in renal cell carcinoma

作　　者：司彤[1] 刘春晓[1] 许凯[1] 孙丰浩[2] 张士强[3] 桂耀庭[4] 

机构地区：[1]南方医科大学珠江医院,广州510282 [2]汕头大学医学院 [3]安徽医科大学 [4]北京大学深圳医院

出　　处：《山东医药》2012年第22期6-8,共3页Shandong Medical Journal

基　　金：广东省深圳市科技计划项目(201003099)

摘　　要：目的观察肾细胞癌组织中DNA拓扑异构酶2α(TOP2α)mRNA、蛋白的表达变化,并探讨其意义。方法采用RT-PCR法检测36例肾细胞癌及癌旁正常组织TOP2αmRNA,用免疫组化SP法检测40例肾细胞癌、19例癌旁正常组织中TOP2α蛋白。结果 TOP2α在肾细胞癌和癌旁组织中均有不同程度的表达,但在肾细胞癌的表达显著高于癌旁正常组织(P<0.05)。TOP2α的表达与肿瘤浸润程度成正相关(P<0.05),而与年龄、性别、淋巴结转移、远处转移及复发等无统计学关联(P>0.05)。结论肾细胞癌组织中TOP2αmRNA、蛋白的表达明显高于癌旁正常组织,且与原发肿瘤浸润程度呈正相关。TOP2αmRNA、蛋白的过表达可能促进肾细胞癌的进展。Objective To investigate the expression of DNA topoisomerase 2 alpha （Top2α） mRNA and protein in renal cell carcinoma,and explore its significance. Methods Real-time quantitative polymerase chain reaction （RT-PCR） was used to detect the expression of Top2α mRNA in 36 cases of renal cell carcinoma and normal adjacent tissues. Immu- nohistochemical SP method was used to detect Top2α protein in 40 renal cell carcinoma a 19 normal adjacent tissues. Re- suits Expression of Top2α can be detected in both tissues. But the expression rate of Top2α in renal cell carcinoma was significantly higher than that in normal tissues （ P 〈 0.05 ）. The expression of Top2α in renal cell carcinoma and T stage was positively correlated（ P 〈 0.05 ）. However, it showed no significant difference between age, gender, lymph node metastasis, metastasis, recurrence（P 〉0.05 ）. Conclusion The expression of Top2α mRNA and protein increase in renal cancer than in normal adjacent tissues, and that was positively correlated with primary tumor invasion. The over-expression of Top2α may contribute to the progression of renal cell carcinoma.

关 键 词：肾肿瘤 肾细胞癌 DNA拓扑异构酶 

分 类 号：R737.1[医药卫生—肿瘤]