卵巢癌细胞顺铂耐药相关的转录因子表达分析  

Analysis on the potential transcriptional factors associated with cisplatin resistant in ovarian cancer cells

作　　者：孙蓬明 宋一一[1] 毛晓丹[1] 董滨华[1] 陈小芳[1] 林芬[1] 林超琴[1] 

机构地区：[1]福建医科大学教学医院福建省妇幼保健院妇科肿瘤研究室,福州350001

出　　处：《福建医药杂志》2012年第3期1-5,共5页Fujian Medical Journal

基　　金：福建省科技厅重点资助项目(2009Y0007)

摘　　要：目的分析并比较卵巢癌细胞中顺铂耐药的相关转录因子。方法采用345通道的高通量转录因子活性检测芯片分析并比较卵巢癌细胞系SKOV3及其顺铂耐药细胞株SKOV3-DDP中转录因子的活化情况,明确差异性表达的转录因子。并采用ELISA方法验证芯片检测结果。结果在345个候选转录因子中共筛选出等与DDP耐药相关的53个表达差异的转录因子。其中卵巢癌耐药株SKOV3-DDP细胞比SKOV3细胞转录因子活性表达上调的有31种,下调的有22种。结论转录因子芯片是筛选、研究卵巢癌致病机制中主要转录因子的先进手段。转录因子可能参与了卵巢癌耐药的相关机制。Objective To analyse the potential transcriptional factors associated with cisplatin chemotherapy resistant. Methods We used ovarian cancer ceil line SKOV3 and its cisplatin resistant cells SKOV3-DDP as a pair of drug sensitive and drug resistant in vivo cellular model. The cellular apoptosis of SKOV-3 and SKOV3-DDP was compared by flow cytometer after treatment with 2-8 mg/mL concentration of cisplatin. Furthermore, 345-channel Protein/DNA array was used to analyse the potential transcriptional factors with cisplatin related resistant in these celt lines. Results In total 345 candidate transcriptional {actors, 53 transcriptional factors were associated with cispiatin related resistant. Compared with SKOV3 cell, the 31 tran- scriptional factors activities were up-regulation and 22 were down-regulation in SKOV3-DDP cell. Conclusion Transcriptional factors microarray is a useful tool to screen the potential transcription factors associated with cisplatin resistant in ovarian cancer.

关 键 词：卵巢癌 顺铂 耐药 转录因子 芯片 转录活性 

分 类 号：R737.31[医药卫生—肿瘤] R963[医药卫生—临床医学]