腹腔镜胃癌手术前后腹腔冲洗液survivin的检测  

Detection of survivin in peritoneal lavage fluid before and after laparoscopic radical gastrectomy

作　　者：刘宏斌[1] 柳万忠[2] 苏琳[1] 韩晓鹏[1] 李洪涛[1] 赵青川[3] 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050 [2]兰州大学第二临床医学院 [3]第四军医大学西京消化病医院

出　　处：《腹腔镜外科杂志》2012年第6期401-404,共4页Journal of Laparoscopic Surgery

基　　金：甘肃省自然科学基金资助项目(编号:2009GS02430)

摘　　要：目的:探讨腹腔镜胃癌根治术对腹腔癌细胞脱落的影响。方法:收集50例胃癌患者的腹腔冲洗液,采用流式细胞术检测胃癌患者腹腔镜手术前后腹腔冲洗液存活素(survivin)的表达情况,并采用薄层液基细胞制片术进行腹腔冲洗液细胞学检查(pleural lavage cytology,PLC)。结果:50例患者腹腔镜胃癌手术前腹腔冲洗液中survivin阳性表达率为58.0%(29/50),术后阳性率为66.0%(33/50),两者差异无统计学意义(P>0.05)。手术前后PLC阳性率分别为22.0%(11/50)和28.0%(14/50)(P>0.05)。术前、术后PLC阳性患者survivin均为阳性。结论:腹腔镜胃癌根治手术未增加腹腔胃癌细胞的脱落。但由于实验样本数量较少,尚需进一步通过长期随访及多中心研究证实。Objective：To explore the effect of laparoscopic radical gastrectomy on free cancer cells in peritoneal lavage fluid. Methods ： Survivin was detected in peritoneal lavage fluid from 50 patients with gastric cancer before and after laparoscopic radical gastrectomy using flow cytometry （FCM）. The HE staining and peritoneal lavage cytology （ PLC ） were performed by thinprep cytologic test. Results：There were no significant differences in the positive rates of survivin before and after surgery （58.0% and 66.0%, respectively）. The preoperative and postoperative PLC positive rates were 22.0% （11/50） and 28.0% （14/50）. The results of FCM were positive in patients with positive preoperative and postoperative PLC. Conclusions： Laparoscopic radical gastrectomy does not increase the amount of intraperitoneal free cancer cells. However, this conclusion needs to be confirmed by more studies.

关 键 词：胃肿瘤 腹腔冲洗液 SURVIVIN 腹腔镜检查 

分 类 号：R735.2[医药卫生—肿瘤]