GATA4基因修饰骨髓间充质干细胞对心肌梗死大鼠心功能的影响  

Effect of mesenchymal stem cells modified by GATA4 on heart function in rats with myocardial infarction

作　　者：赵东海[1] 罗树立[2] 李大鹏[3] 

机构地区：[1]吉林医药学院病理学教研室,吉林吉林132013 [2]吉林省长春市中心医院胸外科,吉林长春130051 [3]吉林医药学院附属医院呼吸内科,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2012年第3期461-464,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省教育厅"十一五"科学技术研究项目资助课题(2009309)

摘　　要：目的:研究锌指转录因子(GATA4)基因修饰骨髓间充质干细胞(MSCs)对心肌梗死大鼠心功能的影响,为进一步探讨其机制提供依据。方法:构建GATA4-pEGFP基因质粒,体外分离培养大鼠MSCs,利用脂质体将质粒转染入MSCs。40只SD大鼠制备大鼠心肌梗死模型,于术后10d测定心功能指标。将模型鼠随机分为MSCs组和DMEM组,每组各20只。MSCs组将GATA4-pEGFP基因修饰的MSCs植入心肌梗死后10d大鼠心肌梗死区,DMEM组同时注射等体积无血清DMEM于心肌梗死区。移植注射后4周,检测左心室射血分数(LEVF)和左室缩短分数(FS)。结果:体外分离培养的MSCs呈梭形或条形,贴壁生长。GATA4-pEGFP重组基因质粒转染MSCs 2周后,可见细胞中有绿色荧光蛋白表达。移植4周后,MSCs组大鼠LEVF(85%±7%)明显高于DMEM组(47%±4%)(P<0.01);MSCs组大鼠FS(52%±6%)明显高于DMEM组(35%±8%)(P<0.01)。结论:GATA4基因修饰的MSCs移植能够改善心肌梗死大鼠心功能。Objective To investigate the effect of mesenchymal stem cells（MSCs） modified by GATA4-pEGFP gene on heart function in myocardial infarction rats and to provide basis for further research on its mechanism.Methods The MSCs were isolated and cultured in vitro.GATA4-pEGFP plasmid was constructed and transfected into MSCs by liposome.40 SD rats were operated to prepare myocardial infarction models and the heart function indexes of the models were evaluated 10 d after the operation.The model rats were randomly divided into MSCs group（n=20） and DMEM group（n=20）.The MSCs modified by GATA4-pEGFP gene was transplanted into the area of myocardial infarction of rats 10 d after infarction in MSCs group.The equal volume of serum-free DMEM was injected into the myocardial infarction area of rats at the same time in DMEM group.The left ventricular ejection fraction（LVEF） and fractional shortening（FS） 4 weeks after injecting were detected.Results The isolated MSCs in vitro adhered to the plate and they presented fusiform or spindle shape.2 weeks after transfection with recombinant gene plasmid GATA4-pEGFP,the green fluorescence protein expressed in MSCs.4 weeks after transplantation,the LEVF value in MSCs group（85%±7%） was increased significantly compared with DMEM group（47%±4%）（P〈0.01）,and the FS value in MSCs group（52%±6%） was also increased significantly compared with DMEM group（35%±8%）（P〈0.01）.Conclusion The heart function of myocardial infarction rat can be improved by transplanting MSCs modified by GATA4 gene.

关 键 词：GATA4基因 骨髓间充质干细胞 心肌梗死 心功能 

分 类 号：Q26[生物学—细胞生物学] R361.3[医药卫生—病理学]