Twist1蛋白在胃癌组织中的表达及其临床意义  

Expression of Twist1 protein in gastric carcinoma tissues and its clinical significance

作　　者：孙凤丹[1] 崔演[2] 张金子[1,3] 程志芬[1] 玄延花[1] 

机构地区：[1]延边大学医学院病理学教研部,吉林延吉133002 [2]延边大学附属医院肿瘤科,吉林延吉133000 [3]吉林博华医院内科,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2012年第3期528-531,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30960403;81160315);吉林省自然科学基金资助课题(201215238);延边大学科技发展计划项目资助课题(2011-53)

摘　　要：目的:检测Twist1蛋白在胃癌组织中的表达,探讨其在胃癌发生、发展过程中的作用及其临床病理学意义。方法:采用免疫组织化学法检测Twist1蛋白在199例胃癌、25例高级别上皮内瘤变、24例低级别上皮内瘤变、46例肠上皮化生、17例慢性萎缩性胃炎和23例正常胃黏膜组织中的表达水平,分析Twist1蛋白表达与胃癌临床病理学特征之间的关系。结果:Twist1蛋白在胃癌组织中的表达明显高于上皮内瘤变、肠上皮化生、慢性萎缩性胃炎和正常胃黏膜组织,差异有统计学意义(P<0.001)。胃癌组织中Twist1蛋白的表达水平在肿瘤直径≥4cm组明显高于肿瘤直径<4cm组(P<0.01);有淋巴结转移组明显高于无转移组(P<0.01);死亡组明显高于生存组(P<0.01)。但Twist1蛋白的表达水平与胃癌类型、患者年龄、肿瘤发生部位、T分级、肿瘤分化、临床分期和肿瘤组织学类型无统计学关联(P>0.05)。结论:Twist1蛋白在胃癌组织中高表达,并与肿瘤大小、转移情况和患者预后有关,其可能是胃癌发生的一个重要因素。Objective To detect the expression of Twist1 protein in gastric carcinoma tissues,and to explore the clinicopathological significance of Twist1 protein in the carcinogenesis and progression of gastric carcinoma.Methods The expressions of Twist1 protein were detected by immunohistochemistry in 199 cases of gastric carcinomas,25 cases of high-grade intraepithelial neoplasia,24 cases of low-grade intraepithelial neoplasia,46 cases of intestinal metaplasia,17 cases of chronic atrophic gastritis and 23 cases of normal gastric mucosa.The relationships between the expression of Twist1 protein and clinicopathological characteristics of gastric carcinoma were analyzed.Results The expression of Twist1 protein in gastric carcinoma tissue was higher than those in intraepithelial neoplasia,chronic atrophic gastritis and normal gastric mucosa tissues,and there were significant differences（P〈0.001）.Meanwhile,the expression of Twist1 protein in tumor size ≥4 cm group was higher than that in tumor size 〈4 cm group（P〈0.01）,and it was higher in the patients with lymph node metastasis than that in patients without metastasis（P〈0.01）,and it was higher in the died patients than that in lived patients（P〈0.01）.However,the expression of Twist1 protein was not correlated with the type of gastric carcinoma,patient’s age,tumor location,T stage,tumor differentiation,clinical stage and histological type of gastric carcinoma.Conclusion The expression of Twist1 in gastric carcinoma tissue is high,and it is related to tumor size and metastasis and prognosis,which may be an important factor in occurence of gastric carcinoma.

关 键 词：胃肿瘤 Twist1蛋白 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]