塞来昔布对乳腺癌阿霉素耐药细胞株MCF-7/ADM的增殖抑制作用  

Inhibitory effect of celecoxib on proliferation of adiramycin-resistant breast cancer cells MCF-7/ADM

作　　者：哈森高娃 王杨[2] 楚小慧[2] 

机构地区：[1]内蒙古林业总医院血液肿瘤科,内蒙古牙克石022150 [2]吉林大学中日联谊医院特需病房,吉林长春130033

出　　处：《吉林大学学报（医学版）》2012年第3期559-562,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅白求恩医学专项基金资助课题(200705290)

摘　　要：目的:通过研究选择性环氯化酶2(COX-2)抑制剂塞来昔布联合阿霉素(ADM)对乳腺癌MCF-7/ADM细胞株生长的作用,探讨塞来昔布的抗肿瘤作用。方法:MCF-7/ADM细胞加入不同浓度等倍稀释的ADM(0.05、0.10、0.20、0.40、0.80和1.60mg.L-1)为对照组,MCF-7/ADM细胞加入无细胞的完全培养基为阴性对照组,在对照组的基础上联合应用10、20μmol.L-1塞来昔布为实验组,各组细胞于24、48、72h后采用CCK-8检测细胞生长抑制率。MCF-7/ADM细胞单加0.05mg.L-1 ADM及联合塞来昔布(10、20μmol.L-1)处理后3h收集细胞,采用流式细胞术检测细胞内ADM水平。结果:不同浓度ADM单药及联合塞来昔布(10和20μmol.L-1)作用于MCF-7/ADM 24、48和72h后细胞生长受到抑制,实验组不同时间细胞生长抑制率均高于对照组(P<0.05),且20μmol.L-1塞来昔布实验组细胞生长抑制率高于10μmol.L-1塞来昔布实验组,差异具有统计学意义(P<0.05)。对照组24、48和72h的细胞未见明显变化,但实验组细胞作用48和72h后出现细胞改变及死亡,呈塞来昔布剂量依赖性。与对照组比较,塞来昔布实验组(10和20μmol.L-1)细胞内ADM浓度升高(P<0.05);20μmol.L-1塞来昔布实验组细胞内ADM浓度高于10μmol.L-1塞来昔布实验组(P<0.05)。结论:选择性COX-2抑制剂塞来昔布联合ADM可有效逆转乳腺癌ADM细胞株耐药。Objective To study the inhibitory effect of celecoxib on proliferation of adiramycin（ADM）-resistant breast cancer cells MCF-7/ADM and to discuss the anti-tumor effect of celecoxib.Methods The MCF-7/ADM cells treated with different concentrations of ADM（0.05,0.10,0.20,0.40,0.80,1.60 mg·L-1） were used as control groups;the MCF-7/ADM cells treated with cell-free complete medium were used as negative control group and the cells treated with ADM and 10,20 μmol·L-1 celecoxib were used as experiment group.The inhibitory rates of cells in various groups were detected by CCK-8 after 24,48 and 72 h.The MCF-7/ADM cells treated with 0.05 mg·L-1 ADM and different concentrations of celecoxib（10 and 20 μmol·L-1） were collected 3 h after treatment,the concentrations of ADM were detected by flow cytometry in various groups.Results The growth of cells were inhibited 24,48 and 72 h after treated with different concentrations of ADM alone or combined with celecoxib（10 and 20 μmol·L-1）.The inhibitory rates of cells in experiment group were higher than those in contronl group（P〈0.05）;the inhibitory rates in 20 μmol·L-1 celecoxib experiment group were higher than those in 10 μmol·L-1 celecoxib experiment group at 24,48 and 72 h（P〈0.05）.The cells in control groups changed little but the cells in experiment group changed a lot,which showed a celecoxib dose-dependent manner.Compared with control groups,the concentrations of ADM in the cells in experiment group were increased（P〈0.05）.The concentration of ADM in the cells in 20 μmol·L-1 celecoxib experiment was higher than that in 10 μmol·L-1 celecoxib eperiment group（P〈0.05）.Conclusion The selective COX-2 inhibitor celecoxib combined with ADM can reverse the ADM resistance of breast cancer cell lines.

关 键 词：塞来昔布 乳腺肿瘤 乳腺癌细胞 MCF-7/ADM 阿霉素 

分 类 号：R737.9[医药卫生—肿瘤]