组织蛋白酶B在膀胱移行细胞癌中的表达及意义  

xpression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance

作　　者：石涛[1,2] 高艳[1,3] 郭永连[1,2] 

机构地区：[1]襄阳市第一人民医院 [2]湖北医药学院附属襄阳医院泌尿外科,湖北襄阳441000 [3]湖北医药学院附属襄阳医院贵宾科,湖北襄阳441000

出　　处：《华西医学》2012年第6期892-894,共3页West China Medical Journal

摘　　要：目的探讨组织蛋白酶B(CB)在膀胱移行细胞癌(TCC)中的表达以及其与TCC浸润的关系。方法取TCC标本40例,TCC分级Ⅰ级23例,Ⅱ～Ⅲ级17例;表浅型TCC(Tis,Ta,T1期)25例,浸润型TCC(T2～4期)15例。另取10例正常膀胱组织作为对照。用链霉素抗生物素蛋白-过氧化物酶连接法行CB免疫组织化学染色观察并计算CB阳性细胞百分率。结果正常膀胱组织中基质无明显着色;在TCC癌组织中,CB可为细胞染色,部分基质亦有染色,部分毛细血管内皮细胞及部分成纤维细胞CB表达阳性,在癌周血管内皮细胞的阳性着色CB表达增强。CB在分级和分期高的癌组织中多为弥散阳性染色。TCC分级Ⅰ级组、TCC分级Ⅱ～Ⅲ级组、TCC分期表浅型组、TCC分期浸润型组及正常对照组的CB阳性细胞百分比分别为10.53%±3.76%、21.52%±3.58%、11.32%±2.69%、20.57%±3.25%、0.11%±0.18%,TCC各组均高于正常对照组(P<0.01);TCC分级Ⅱ～Ⅲ级组高于TCC分级Ⅰ级组,TCC分期浸润型组高于TCC分期表浅型组,差异均有统计学意义(P<0.01)。结论 CB可能成为判断TCC进展和预后的重要指标。Objective To investigate the expression of cathepsin B （CB） in the bladder transitional cell carcinoma （TCC） tissues and the relation between it with the invasion of TCC. Methods Forty samples of TCC including 23 of grade Ⅰ and 17 grade Ⅱ to Ⅲ were studied. Among them, 25 were superficial type （Tis, Ta, T1）, and 15 were invasion type （T2-4）. Another 10 normal samples were designated as the control group. Immunohistochemical staining of CB in TCC and normal tissues was carried out by the streptavidin-biotin method. Results There were no apparent coloring in the normal bladder tissue ground substance. In TCC tissues, CB could dye the cells and a portion of ground substance. CB was positive in part of the capillary endothelial cells and fibroblasts, and in peritumoral vascular endothelial cells, the positive expression of CB was enhanced. Positive diffusion staining existed in high grade and high stage cancers. The proportions of CB staining cells for grade Ⅰ cancer tissues, grade Ⅱ -Ⅲ cancer tissues, TCC superficial type of tissues, TCC invasion type of tissues and normal tissues were respectively 10.53%± 3.76%, 21.52% ±3.58%, 11.32% ±2.69%, 20.57%± 3.25%, and 0.11% ±0.18%. The mean value of cells of CB staining was significantly higher in cancer tissues than in normal tissues （P 〈 0.01）. The mean value of positive CB staining in grade Ⅱ-Ⅲ cancer tissues was significantly higher than that in grade Ⅰ cancer tissues （P 〈 0.01）. The mean value of posive CB staining in stage T2-4 cancer tissues was significantly higher than that in superficial stage Tis-1 cancer tissues （P 〈 0.01）. Conclusion CB should become a important marker to determine TCC development and its prognosis.

关 键 词：组织蛋白酶B 膀胱移行癌细胞 表达 

分 类 号：R737.14[医药卫生—肿瘤]