附子多糖诱导肝癌患者外周血树突状细胞分化成熟的实验研究  

Induction of Differentiation of Dendritic Cells Derived from Hepatocellular Carcinoma by Fuzi Polysaccharides

作　　者：高林林[1] 曾升平[1] 潘力弢[2] 

机构地区：[1]成都中医药大学临床医学院,成都市610072 [2]深圳市第二人民医院中医科

出　　处：《中国肿瘤临床》2012年第13期882-885,894,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨附子多糖是否能在体外诱导肝癌患者外周血单核细胞向树突状细胞分化并成熟表达细胞表面分子,为进一步研究附子多糖抗肿瘤作用机制奠定基础。方法:取肝癌患者外周血体外分离单个核细胞,去除淋巴细胞后进行培养,实验组加入低、中、高3种不同浓度的附子多糖,另设加入GM-CSF、IL-4和TNF-α的阳性对照组和不加任何诱导剂的阴性对照组,共培养10d。其间进行细胞计数、倒置显微镜下动态观察细胞形态、MTT法细胞活力检测、扫描电镜细胞成像、流式细胞仪检测细胞表型(CD80、CD83、CD86)等检测。结果:中浓度附子多糖组能够诱导肝癌患者外周血单核细胞分化为树突状细胞,并促进细胞增殖,高度表达CD80、CD83、CD86等共刺激分子和表面标记物,与阳性对照组相比,两组结果比较无明显差异,与高、低浓度附子多糖组和阴性对照组结果相比有显著差异。结论:适当浓度附子多糖能够在体外有效诱导肝癌患者外周血单核细胞分化为树突状细胞并表达成熟表型,从而作为第二信号活化T淋巴细胞,激发肿瘤免疫。To investigate whether Fuzi polysaccharice（FPS）induce the differentiation of Peripheral blood mononuclear cells to dendritic cells （ DC ）. Methods： Lymphocytes are removed form the monocytes derived from peripheral blood of patients with hepatocellular carcinoma.High concentration,medium concentration and low concentration of PBS were added into test groups, GM-CSF, IL-4, TNF-αwere added into the positive contrast group and nothing were added into in negative contrast group.In ten days, the amounts of cells were caculated, the shape of cells were observed dynamiely by inverted microscope, cells were scanned by scaning electron microscope, and the surface types of DC（ CD80, CD83, CD86 ） were tested by flow cytometer. Results： Medium concentration group of FPS could induce the differentiation of Peripheral blood mononuclear cells to DC, promote the proliferation of DCs and the expression of CD80, CD86 and CD83. These are no difference between positive contrast group and medium concentration group of FPS.But there are Significantly difference from medium concentration group of FPS to high concentration group of FPS, low concentration group of FPS and negative contrast group. Conclusion： FPS could induce the differentiation of Peripheral blood mononuclear cells to dendritic cells （ DC ）, promote the proliferation of DC and the expression of mature surface molecule.

关 键 词：附子 多糖 分化 肝癌 树突状细胞 

分 类 号：R735.7[医药卫生—肿瘤]