秦皮素抑菌活性及其机制研究  

Antibacterial activity of fraxetin and its mechanism

作　　者：杨春雪[1] 汪业菊[1] 谢明杰[1] 

机构地区：[1]辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室,大连116021

出　　处：《免疫学杂志》2012年第8期703-705,共3页Immunological Journal

基　　金：辽宁省教育厅项目(L2010236);大连市计划项目(2009E12SF165)

摘　　要：目的以金黄色葡萄球菌为供试菌株来研究秦皮素对其的抑制活性及其机制。方法利用牛津杯法、2,3,5-氯化三苯基四氮唑(TTC)染色、生长曲线的测定、细胞膜渗透性测定、SDS-PAGE蛋白谱变化方法等对秦皮素的抑菌活性及其机制进行研究。结果秦皮素能够影响金黄色葡萄球菌细胞膜的通透性,秦皮素作用20 h,菌体可溶性蛋白总量减少55.74%。结论秦皮素对金黄色葡萄球菌具有明显的抑制作用,其抑菌机制可能是通过抑制蛋白质的合成来实现的。To investigate the antibacterial activity oi fraxetin on Staphylococcus aureus and its mechanism,we employed 2, 3, 5-triphenyl tetrazolium chloride （TTC） staining, and determined membrane penetrability and the change of SDS-PAGE protein spectra. Fraxetin could impact on the membrane permeability of Staphylococcus aureus, but not destroy the membrane integrity directly. After treated with fraxetin for 20 hours, the total content of proteins decreased to 55.74%. The results suggested that fraxetin has obvious antibacterial activity on Staphylococcus aureus, and the antibacterial mechanism of fraxetin may be achieved by inhibition of protein synthesis.

关 键 词：秦皮素 抑菌活性 可溶性蛋白 

分 类 号：R285[医药卫生—中药学]