黑根霉RN-M246催化甾体的11α-羟基化培养基研究  

Optimization of Fermentation Medium for 11α-Hydroxylation of Steroid Catalyzed by Rhizopus Nigricans RN-M246

作　　者：胡曦予[1] 崔励[1] 冯魁[1] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《化学与生物工程》2012年第6期77-79,共3页Chemistry & Bioengineering

摘　　要：应用微生物转化法,以黑根霉RN-M246催化底物雄甾-4-烯-3,17-二酮的11α-羟基化反应,考察了发酵培养基对转化的影响,分别从碳源、速效有机氮源、迟效氮源、无机氮源及pH值5个方面优化了发酵培养基。确定优化的发酵培养基(g·L-1)为:葡萄糖30、蛋白胨20、冷榨豆粉10、柠檬酸三铵1、磷酸氢二钾5、pH值5.0,在优化条件下,可提高转化率约13%,最终转化率达48.4%。The microbial 11α-hydroxylation of androst-4-ene-3,17-dione was catalyzed by Rhizopus nigri- cans RN-M246 and the effect of fermentation medium on the transformation was investigated. The medium was optimized in five factors including carbon source, fast acting nitrogen source,slow release nitrogen source,inor- ganic nitrogen source and pH value. The optimal medium was determined as follows：glucose 30 g . L^-1 ,peptone 20 g. L^-1 ,cold pressed soybean cake powder 10 g . L^-1~ ,triammonium citrate 1 g . L^-1 , dipotassium hydrogen phosphate 5 g . L^-1 ,pH value 5.0. Under above conditions,the conversion rate increased by about 13% and the final conversion rate was 48.40%.

关 键 词：微生物转化 黑根霉 雄甾-4-烯-3 17-二酮 11α-羟基化反应 

分 类 号：TQ920[轻工技术与工程—发酵工程]