P16,Ki67与HPV16/18在宫颈病变中的表达及其临床意义  

Expression of P16,Ki67 and HPV16/18 in cervical lesions and their clinical implications

作　　者：杨东杰[1] 徐秋岚[1] 茅青[1] 

机构地区：[1]江苏省无锡市第三人民医院病理科,江苏无锡214041

出　　处：《国际病理科学与临床杂志》2012年第3期211-215,共5页Journal of International Pathology and Clinical Medicine

摘　　要：目的:研究多肿瘤抑制基因P16、细胞核增殖抗原Ki67及高危型人乳头状瘤病毒(high riskhuman papilloma virus,HPV16/18)在宫颈病变中的表达和临床意义。方法:采用免疫组织化学Elivision两步法检测P16和Ki67在宫颈非特异性炎症、宫颈上皮内瘤变(cervical intraepithelialneoplasia,CIN)I级、Ⅱ级和Ⅲ级及宫颈鳞状细胞癌中的表达,用原位杂交法检测HPV16/18的表达。结果:P16蛋白表达在非特异性炎症、CINⅠ级、Ⅱ级和Ⅲ级及宫颈癌中的阳性率分别为20.0%,55.6%,93.6%和100.0%;Ki67蛋白表达在非特异性炎症、CINⅠ级、Ⅱ级、Ⅲ级及宫颈癌中的阳性率分别为17.6%,61.1%,89.4%和100.0%,不同组别间两两比较差异均有统计学意义(P<0.05)。而且P16和Ki67的阳性表达率及染色强度呈递增趋势,表达存在分层现象。HPV16/18在非特异性炎症、CINⅠ级、Ⅱ级、Ⅲ级及宫颈癌中的阳性率分别为30.0%,61.1%,82.9%和100.0%,宫颈病变中HPV16/18的阳性率显著高于非特异性炎症组。结论:P16和Ki67蛋白检测作为宫颈病变的有效的生物学标记,可联合HPV16/18 DNA检测应用于宫颈癌及其癌前病变的筛查和诊断中。Objective： To explore the expression of multiple tumor suppressor gene P16,proliferating cell nuclear antigen Ki67, and high risk human papilloma virus （HPV16/18） in cervical lesions and their clinical implications. Methods： Iimmunohistochemistry was used to examined the expression of P16 and Ki67 in cervical inflammatory lesions, cervical intraepithelial neoplasia （CIN） Ⅰ, Ⅱ, Ⅲand squamous cell carcinoma. HPV16/18 was detected by hybridization in situ. Results： P16 expression was confirmed in 20.0%,55.6%,93.6%, and100.0% of inflammatory lesions, CINⅠ, Ⅱ, Ⅲand squamous cell carcinoma, respectively. Positive expression of Ki67 was observed in 17.6%,61.1%,89.4%, and 100.0% of inflammatory lesions, CINⅠ, Ⅱ, Ⅲand squamous cell carcinoma, respectively. There were significant differences in P16 and Ki67 among the various groups （PConclusion： P16 and Ki67 proteins are useful as biological markers of cervical lesions; combined with HPV16/18 DNA detection, they can be helpful in screening and diagnosing cervical cancer and precancerous lesions.

关 键 词：宫颈上皮内瘤变 鳞状细胞癌 P16 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]