脂质体rIL-2和肿瘤浸润淋巴细胞治疗鼠H22肝癌的体内实验研究  

Experimental Study of Treating Mice H22 Hepatoma Using Liposomal- rIL- 2 and TIL

作　　者：于静[1] 赵治国[2] 卢玫 段芳龄[2] 

机构地区：[1]河南省人民医院二内科,郑州市450003 [2]河南医科大学第二附属医院消化科 [3]日本中外制药北京事务所

出　　处：《中国肿瘤临床》2000年第4期297-299,共3页Chinese Journal of Clinical Oncology

基　　金：河南省教委科研基金

摘　　要：目的 :比较脂质体rIL - 2和游离rIL - 2分别联合肿瘤浸润淋巴细胞治疗鼠H2 2肝癌的效果及副作用。方法 :采用薄膜分散法和超声波粉碎处理法制备脂质体rIL - 2 ;选昆明鼠 40只 ,随机分组进行体内治疗 ,游离rIL - 2用量为脂质体rIL - 2的 3倍 ,观察瘤出现时间、瘤体积、瘤湿重及鼠体温的变化。结果 :TIL +rIL - 2组的瘤出现时间、瘤体积、瘤湿重分别为 :15± 2 .5d、2 .44± 2 .97cm3 、4.7± 2 .0 g ;TIL +脂质体rIL - 2组分别为 :15± 3.2d、2 .2 7± 2 .5 8cm3 、4.5± 2 .1g ;二者与对照组比较均显出了肯定的治疗效果 (P <0 .0 5 ) ,但二者之间比较无统计学差别 (P >0 .0 5 )。TIL +rIL - 2组有明显发热反应 ,TIL +脂质体rIL - 2组体温正常。结论 :脂质体rIL - 2较游离rIL - 2抗肿瘤作用提高 ,副作用降低。Objective: To compare the effect and side-effect of liposomal-rIL-2 and free rIL-2 in the treatment of mice H22 hepatoma. Methods: After preparing liposomal-rIL-2 by membrane disperse and ultrasonic crush, 40 mice with hepatoma were randomly divided into 4 groups and treated with liposomal-rIL-2+TIL or rIL-2+TIL and the dose of rIL-2 was three times of liposomal-rIL-2. Results: In TIL+rIL-2 group, the tumor occuring time, tumor size and tumor weight were 15±2.5d, 2.44±2.97cm 3 and 4.7±2.0g respectively. In TIL+liposomal-rIL-2 group, 15±3.2d, 2.27±2.58cm 3 and 4.5±2.1g respectively. As compared to control, the effect of both on antitumor action was positve ( P <0.05), but there was no statistical significane between these two groups ( P >0.05). The termperature of TIL+rIL-2 group mice was higher than that of TIL+liposomal-rIL-2 group mice ( P <0.05). Conclusion: The antitumor action of liposomal-rIL-2 is higher and the side effect is lower than those of rIL-2.

关 键 词：脂质体 RIL-2 肿瘤浸润淋巴细胞 H22肝癌 

分 类 号：R735.7[医药卫生—肿瘤]