乳腺癌中郑氏植物蛋白的表达及其与Her-2基因扩增的关系  

Expression of ZPPS in human breast carcinoma and its relationship with Her-2 gene amplification

作　　者：陈琛[1] 何春年[1] 杨仕海[2] 徐明堂[1] 袁艳龙[1] 

机构地区：[1]河北省人民医院病理科,石家庄050051 [2]河北省儿童医院心外科,石家庄050031

出　　处：《临床与实验病理学杂志》2012年第7期749-752,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省卫生厅中医药管理局基金(2006067)

摘　　要：目的检测郑氏植物蛋白片段(Zheng’s plant protein segment,ZPPS)在乳腺癌中的表达,探讨其临床病理联系及其与Her-2基因表达的相关性。方法应用免疫组化SP法检测51例乳腺癌与23例正常乳腺组织中ZPPS的表达;应用FISH技术检测Her-2基因在乳腺癌中的表达。结果正常乳腺组织中,8例ZPPS呈阳性表达,阳性率为34.78%;乳腺癌组织中,38例ZPPS呈阳性表达,阳性率为74.51%,二者表达差异有统计学意义(P<0.05)。51例乳腺癌中Her-2基因扩增率为39.22%,ZPPS阳性表达与FISH检测Her-2基因扩增呈负相关(P<0.05)。ZPPS的表达与淋巴结转移呈负相关(P<0.05)。结论标记ZPPS对乳腺癌的诊断及判定淋巴结转移具有实用价值。因在乳腺癌中ZPPS的表达与Her-2基因扩增呈负相关,所以临床病理工作中可选择ZPPS阴性病例进行FISH检测,从而减少盲目进行FISH基因扩增和Herceptin分子靶向治疗的病例。Purpose To detect the expression of Zheng＇ s plant protein segment （ZPPS） in breast invasive carcinoma, and to evaluate the relationship of ZPPS＇ s expression in breast invasive carcinoma with clinicopathological parameters and Her-2 gene amplification. Methods The expression of ZPPS was detected in normal breast tissue and breast invasive carcinoma by immunohistochemistry （1HC） in breast carcinoma. The expression of Her-2 gene was detected in breast invasive carcinoma by fluorescence in situ hybridization （FISH）. Results The expression of ZPPS was much higher in invasive carcinoma（74.51% ） than that in normal breast tissues （34. 78% ）. Twenty of fifty-one （39. 22% ）cases of breast cancer showed positive for HER-2 gene amplification. The expression of the ZPPS had a significant correlation with Her-2 gene （ P 〈 0.05 ）. And there was a correlation between the expression of ZPPS and the lymph node metastasis （ P 〈 0. 05 ）. Conclusions ZPPS may play a key role in diagnosing and judging lymph node metastasis in breast invasive carcinoma. The negative relationship between expression of ZPPS with Her-2 gene may suggest Her-2 gene amplification is de- tection by FISH in the breast cancer with negative ZPPS expression, which to reduce the detection of Her-2 gene and rationally select the cases for molecular targeting therapy of Herceptin in breast cancer.

关 键 词：乳腺肿瘤 郑氏植物蛋白 HER-2 荧光原位杂交 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]