悬浮培养在肿瘤干细胞研究中的应用及局限性  

The application and limitation of suspension culture in the study of neoplastic stem cells

作　　者：赵笛[1] 郑青[1] 

机构地区：[1]上海交通大学医学院附属仁济医院上海市消化疾病研究所,上海200001

出　　处：《肿瘤》2012年第7期559-563,共5页Tumor

基　　金：上海市科委基础研究重点项目(编号:10JC1419000)

摘　　要：肿瘤干细胞被认为是肿瘤起源、生长和转移的关键因素,已成为当下肿瘤研究的热点之一。由于大多数肿瘤干细胞缺乏特异性的标志物,且组织定位和形态特征不明确,因此无法直接从肿瘤细胞中分离,当前常利用肿瘤干细胞与普通肿瘤细胞功能上的差异区分这2类细胞。悬浮培养法是常用的组织干细胞及肿瘤干细胞的体外研究方法,它能够直观地在单细胞层面上反映细胞的自我更新及增殖能力,因此被广泛用于肿瘤干细胞的鉴定、富集和纯化。然而,悬浮培养获得的细胞与体内真正的肿瘤干细胞存在本质的差别,获得的肿瘤球并不一定具克隆性,且并不是所有具备干细胞性质的细胞都能在悬浮条件下存活。因此,当使用悬浮培养研究肿瘤干细胞时,必须意识到它的局限性,本文旨在对这一问题的研究作一综述。Neoplastic stem cells are generally recognized as a key factor in carcinogenesis and progression and metastasis of cancer. Recently, many studies have been focused on neoplastic stem cells. However, in consideration of lack of specific biological marker and unclear information about the allocation and morphology of neoplastic stem cells, it is difficult to separate them from tumor cells directly. The difference in function between neoplastic stem cells and the tumor cells was commonly used to distinguish them. Suspension culture is one of the most widely used methods to identify, enrich and purify neoplastic stem cells. It can conveniently demonstrate the capabilities of self-renewal and proliferation at the single-cell level. However, the neoplastic stem cells harvested from suspension culture are different from the original neoplastic stem cells, and not all of the cells sharing the features of neoplastic stem cells can survive in the suspension culture. Thus, the limitation of application of suspension culture in the study of neoplastic stem cells should be emphasized, and which is reviewed in this paper.

关 键 词：肿瘤干细胞 细胞培养技术 悬浮培养 肿瘤球 

分 类 号：R733[医药卫生—肿瘤]