防止牙-牙周组织切片脱片技术的改进  

Technological improvement on the prevention of dento-periodontal tissue section from separating from slices

作　　者：章立群[1] 孙辉[1] 邓碧霞[1] 谢安琪[1] 

机构地区：[1]广东医学院附属南山人民医院口腔科,广东深圳518052

出　　处：《海南医学》2012年第13期15-17,共3页Hainan Medical Journal

摘　　要：目的探讨一种可有效防止牙-牙周组织切片脱片的方法。方法改进组采用APES涂胶玻片,在抗原修复前在组织切片上覆盖同等大小纱布和空载玻片,回形针固定。对照组使用普通玻片,烤片过夜,常规脱蜡水化。比较改进组和对照组各40张切片在煮沸法、酶抗原修复后脱片率,并用PCNA检验其染色效果。结果改进组脱片率较对照组明显降低(P<0.01)。煮沸法抗原修复后PCNA染色有核染阳性细胞散在存在于组织中,背景清晰,效果满意。胰酶修复法两组均未出现阳性表达。结论该改进的方法能有效防止牙-牙周组织脱片并不影响染色效果,值得推广应用。Objective To find out an effective way to prevent the dento-periodontal tissue from separating from the slices.Methods In the improvement group,a blank slice and gauze with the same size as the slice were used to cover the tissue,and paperclip was used to clip them together.Ordinary slides were used in the control group.The ratio of tissue separating from the 40 slices of each group was calculated and compared after boiling or enzymatic digestion antigen retrieval method.Immunohistochemical staining of PCNA was used to detect the effect of coloration.Results The ratio of tissue dropping from the slices in the improvement group was lower than that in the control group.PCNA staining also showed positive cell nucleus scattered in the tissues with clear background.No positive cells were seen in the tissue after enzymatic digestion in the two groups.Conclusion The improved technique is helpful in the prevention of dento-periodontal tissue section from separating from slices,with no negative effect on the immunohistochemical results.

关 键 词：抗原修复 牙周组织 免疫组化 

分 类 号：R-332[医药卫生]