硫化氢下调血管紧张素Ⅱ1型受体表达抑制高血压大鼠心肌重构  

Down-regulation of Angiotensin Ⅱ Receptor-1 Expression by Hydrogen Sulfide Inhibits Cardiac Remodeling in Hypertensive Rats

作　　者：黄贤生[1] 王东明[1] 郑锦滨[1] 

机构地区：[1]汕头大学医学院附属第一医院心血管内科,广东省汕头市515041

出　　处：《中国动脉硬化杂志》2012年第9期787-791,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的通过构建大鼠两肾一夹高血压模型(2K1C)造成心肌重构,观察硫化氢是否存在保护效应并初步探索其可能机制。方法 34只健康成年雄性SD大鼠随机分为对照组、假手术组、手术组和硫化氢组,测量实验前后收缩压、左心室质量指数、全心质量指数、左心室心重比,观察心肌细胞直径、横断面面积以及胶原沉积程度,检测心肌细胞血管紧张素Ⅱ1型受体表达情况。结果手术组血压持续增高,左心室质量指数、全心质量指数以及左心室心重比明显增加,镜下观察可见心肌纤维断裂、肌浆肿胀、部分空泡样、颗粒性改变以及灶性坏死、单核细胞浸润。硫化氢组血压呈先升高后下降趋势,左心室质量指数、全心质量指数以及左心室心重比较手术组降低,心肌形态学改变明显减轻。Masson三色染色显示,硫化氢组血管壁渗出明显弱于手术组。手术组血管紧张素Ⅱ1型受体的表达明显上调,硫化氢组血管紧张素Ⅱ1型受体表达显著低于手术组。结论硫化氢能减轻两肾一夹模型大鼠的心肌重构,此作用与下调血管紧张素Ⅱ1型受体蛋白表达有关。Aim To investigate the effect of hydrogen sulfide on cardiac remodeling in rats, after the two-kid- neys-one-clip （2K1 C） model built. Methods 34 male Sprague-Dawley （SD） rats were randomly divided into four groups： control group, sham-operated group, 2K1C model group and hydrogen sulfide （H2S） group. Systolic blood pres- sure （SBP）, the left ventricle weight/body weight （LVW/BW）, the heart weight/body weight （HW/BW） and the left ventricle weight/heart weight （LVW/HW） were measured to assess cardiac remodeling. The diameter, the cross-section- al area of the myocardial cells were evaluated by HE stain. Collagen deposition were detected by Masson stain. Angio- tensin Ⅱ receptor-1 （AT1） expression in the myocardial cells were assessed by immunohistochemistry. Results Compared with the control group, SBP increased persistently in 2K1C group and LVW/BW, HW/BW, LVW/HW in- creased accordingly. While in the H2S group, SBP elevated transiently and tended to decrease afterward. LVW/BW, HW/BW, LVW/HW decreased in H2S group compared with 2K1C group. HE stain showed significantly milder myocar- dial injury in H2S group than that in 2K1C group. Masson stain showed less collagen deposition in H2S group than that in 2K1 C group. The expression of AT1 receptor was up-regulated in the 2K1 C group, which can be reversed by treatment with H2S. Conclusion H2S can attenuate cardiac remodeling in 2K1C rats by down-regulating AT1 expression.

关 键 词：硫化氢 高血压 心肌重构 血管紧张素Ⅱ1型受体 

分 类 号：R363[医药卫生—病理学]