新型多聚体微泡携带舒尼替尼抑制人肾癌GRC-1细胞生长及促凋亡的实验研究  

Study of sunitinib-liposome-loaded microbubbles for growth inhibition and apoptosis induction of renalcarcinoma cell strain GRC-1

作　　者：胡劼[1] 宗瑜瑾[1] 宋宏萍[1] 张景瑶[1] 段艳[1] 张军[1] 周晓东[1] 苏海砾[1] 

机构地区：[1]第四军医大学西京医院超声科,西安710032

出　　处：《中华超声影像学杂志》2012年第7期621-624,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：国家自然科学基金面上项目（81071875）

摘　　要：目的观察新型多聚体微泡携带舒尼替尼对人肾癌GRC一1细胞生长及凋亡的影响。方法将体外培养的人肾癌GRC一1细胞随机分为6组：空白对照组、单纯微泡组、单纯脂质体组、舒尼替尼组、新型多聚体微泡载舒尼替尼不联合超声组、新型多聚体微泡载舒尼替尼联合超声组。MTT法观察不同处理组细胞生存率，Sigma-FITC荧光染色及透射电镜检测细胞凋亡。结果新型多聚体微泡载舒尼替尼联合超声组对人肾癌GRC-1细胞的生长抑制及促进凋亡作用强于其他处理组及对照组。结论新型多聚体微泡携带舒尼替尼在超声作用下对人肾癌GRC-1细胞生长有明显抑制作用，并诱导细胞凋亡。Objective To investigate the effect of growth inhibition and apoptosis induction of sunitinib-liposome-loaded microbubbles on renal carcinoma cell strain. Methods GRC-1 cell strain was cultured in vitro, and was divided into 6 groups：blank control group, pure microbubbles group, pure lipsomes group,sunitinib group, sunitinib-liposome-loaded microhubbles without ultrasound treat group, sunitinih- liposome-loaded microbubbles with ultrasound treat group. Growth inhibition in different groups was observed at different time with MTT assay, apoptosis induction with Sigma-FITC technology and transmission electron microscope. Results The growth inhibition and apoptosis promotion of GRC-1 cell were significantly increased in sunitinib-liposome-loaded microbubbles with ultrasound treat group compared to the other groups. Coneluslons Microbubble guided sunitinib delivery can increase the effect of the growth inhibition and apoptosis induction of GRC-1 cells, which may provide an more effective approach for cancer treatment.

关 键 词：超声检查 微气泡 肾肿瘤 细胞凋亡 舒尼替尼 

分 类 号：R737.11[医药卫生—肿瘤]