桃PpPGIP1启动子的分离与功能分析  

Isolation and Functional Analysis of PpPGIP1 Promoter in Peach

作　　者：王秀云[1] 张计育[1,2] 古咸彬[1] 高志红[1] 章镇[1] 俞明亮[3] 张妤艳[3] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省中国科学院植物研究所,南京210014 [3]江苏省农业科学院园艺研究所,南京210014

出　　处：《园艺学报》2012年第7期1263-1270,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871681,31101526);江苏省科技基础设施建设计划项目(BM2008008)

摘　　要：通过染色体步移法分离桃[Prunus persica(L.)Batch]PpPGIP1 上游启动子序列,利用生物信息学方法对其功能进行初步预测;构建该启动子植物表达载体并通过农杆菌介导法转化烟草,研究不同激素诱导条件下GUS蛋白瞬时表达情况;并利用实时荧光定量 RT-PCR 技术分析了PpPGIP1在水杨酸(SA)、茉莉酸甲酯(MeJA)、脱落酸(ABA)和 1-氨基环丙烷-1-羧酸(ACC)诱导下的表达特性,获得了长度为1018bp的桃PpPGIP1启动子序列。该序列与梅和中国李PGIP启动子序列的同源性分别为90%和89%;该启动子序列含有SA、MeJA、ABA和ETH诱导调控相关的抗病与胁迫顺式作用元件;ABA和ACC可以诱导PpPGIP1启动子调控GUS基因的表达;实时荧光定量RT-PCR分析结果表明:SA、MeJA、ABA和ACC都可以诱导桃叶片中PpPGIP1的表达。PpPGIP1可能参与SA、MeJA、ABA和ETH的信号转导,在桃抵抗病原菌和逆境胁迫方面起着非常重要的作用。Promoter sequence of PpPGIP1 gene in peach[Prunus persica （L.） Batch]was isolated through the chromosome walking method, and its fimction was preliminary forecasted by means of bioinformaties method. The plant expression vector of the promoter was constructed and transformed into tobacco through the Agrobacterium-mediated technique. Expression characteristics of PpPGIP1 gene induced by salicylic acid （SA）, methyl jasmonate （MeJA）, abscisic acid （ABA） and 1-aminocyclopropane- 1-carboxylate （ACC） were also analyzed using real-time RT-PCR. The promoter sequence of PpPGIP1 gene with 1 018 bp length was obtained and its homology with that of Prunus mume and Prunus salicina were 90% and 89%, respectively. The promoter contains important cis-acting elements related to resisting pathogens and adversity stress and induced by SA, MeJA, ABA and ETH. The expression of GUS gene could be regulated by PpPGIP1 promoter with the induction of ABA and ACC. The results of real-time RT-PCR indicate that the expression ofPpPGIP1 gene could be induced by SA, MeJA, ABA andACC in the leaves of peach. Thus, PpPGIP1 gene may be involved in the signal transduction pathway of SA, MeJA, ABA and ETH, and play an important role in the condition of resisting pathogens and adversity stress in peach.

关 键 词：桃 PpPGIP1启动子 调控元件 表达特性 表达载体构建 

分 类 号：S662.1[农业科学—果树学]