Tenascin-C在人原发性肝细胞癌浸润、转移和血管生成中的作用  

Role of Tenascin-C in Invasion,Metastasis and Angiogenesis of Human Hepatocellular Carcinoma

作　　者：尹传昌[1] 廖晓峰[1] 陈雯[2] 郭跃清[1] 易继林[1] 李兴睿[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030 [2]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《华中科技大学学报（医学版）》2012年第3期278-283,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2006ABA117)

摘　　要：目的研究Tenascin-C在原发性肝细胞癌(HCC)中的表达,探讨Tenascin-C与HCC浸润、转移和血管生成的关系。方法采用免疫组织化学方法检测54例HCC、32例肝硬化、17例正常肝组织中Tenascin-C的表达情况以及微血管密度(MVD);采用逆转录聚合酶链反应(RT-PCR)方法检测Tenascin-C mRNA,并结合临床病理指标进行分析。结果 Tenascin-C在HCC、肝硬化和正常肝脏组织中的阳性例数分别为34、6和0,其差异具有统计学意义(χ2=14.06,P<0.01);Tenascin-C在HCC组织内的表达强度明显高于肝硬化组织[(162.15±9.77)vs.(149.24±8.25),t=2.192,P<0.05]。HCC组织Tenascin-C mRNA的表达强度明显高于肝硬化组织[(0.593±0.110)vs.(0.138±0.089),t=2.894,P<0.05]。有包膜浸润的HCC组织中Tenascin-C表达量明显高于无浸润者(t=2.248,P<0.05),Edmondson-Steiner病理分级中HCC属Ⅲ～Ⅳ级的Tenascin-C含量明显高于Ⅰ～Ⅱ级者(t=2.698,P<0.05),HCC有转移的Te-nascin-C含量明显高于无转移者(t=2.785,P<0.01)。HCC组织中Tenascin-C表达与微血管密度(MVD)呈正相关(r=0.68,P<0.05)。获随访的46例(85.2%)HCC患者在随访期间(3～30个月,平均11.5个月),Tenascin-C阴性表达的患者中位生存时间长于Tenascin-C阳性表达的患者[(22.01±6.95)月vs.(15.08±7.06)月,P<0.05,log-rank test]。结论 Tenascin-C对HCC的浸润、转移和血管生成起着重要作用。Tenascin-C和MVD可以作为预测HCC浸润、转移的生物学指标。Objective To investigate the expression of Tenascin-C in human hepatocellular carcinoma（HCC）and its role in HCC invasion,metastasis and angiogenesis.Methods Immunohistochemical staining was employed to detect the expression of Tenascin-C and microvessel density（MVD）in resected specimens of 54 cases of HCC,32 cases of hepatic cirrhosis and 17 cases of normal hepatic tissues.Reverse transcription polymerase chain reaction（RT-PCR）was used to detect Tenascin-C mRNA expression.Results The immunohistochemistry revealed that Tenascin-C was positively expressed in 34 cases of HCC and 6 cases of hepatic cirrhosis tissues,respectively（χ2=14.06,P0.01）.No Tenascin-C was detected in normal hepatic tissues.The immunoreactivity of Tenascin-C in HCC was significantly higher than that in the cirrhosis tissues[（162.15±9.77）vs.（149.24±8.25）,t=2.192,P0.05].The expression of Tenascin-C mRNA in HCC was also significantly higher than that in hepatic cirrhosis tissues[（0.593±0.110）vs.（0.138±0.089）,t=2.894,P0.05].There was a correlation between Tenascin-C expression and capsule invasion（t=2.248,P0.05）,Edmondson-Steiner grade（t=2.698,P0.05）as well as metastasis（t=2.785,P0.01）in HCC.Additionally,MVD of HCC tissues was significantly correlated to the Tenascin-C expression（r=0.68,P0.05）.Forty-six cases of HCC（85.2%）were followed up with a median period of 11.5 months（range 3-30 months）.The Tenascin C-negative patients showed longer median survival time than the Tenascin-positive patients[（22.01±6.95）months vs.（15.08±7.06）months,P0.05,log-rank test].Conclusion Up-regulation of Tenascin-C in HCC is possibly related to tumor invasion,metastasis as well as angiogenesis.Tenascin-C and MVD staining might be used to predict the metastatic and aggressive potential of HCC.

关 键 词：TENASCIN-C 原发性肝细胞癌 微血管密度 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]