实验性即刻再植牙大鼠模型的建立  

Establishment of an animal model for immediate tooth replantation in experimental rat

作　　者：蔡琴[1,2] 姜沙沙[1,3] 王珏[3] 吴叶[2] 周红艳[3] 刘茜[3] 梅予锋[1,3] 

机构地区：[1]南京医科大学口腔医学研究所,江苏南京210029 [2]南京医科大学附属南京第一医院口腔科,江苏南京210006 [3]南京医科大学附属口腔医院儿童口腔预防科,江苏南京210029

出　　处：《口腔生物医学》2012年第2期64-67,共4页Oral Biomedicine

基　　金：江苏高校优势学科建设工程资助项目

摘　　要：目的:建立大鼠即刻再植牙动物模型,观察再植牙的愈合过程与牙根外吸收的表现。方法:30只6周龄SPF级Wist-ar雄性大鼠,分为6组,每组5只。单侧上颌第一磨牙脱位后即刻再植,对侧同名牙为自身对照。分别于术后1、3、7、14、21、28d处死,分离上颌骨;拍根尖X线片,应用图像分析系统测量近中根根尖周阴影面积;标本切片、HE染色,进行组织学观察。结果:近中根根尖周阴影面积随时间延长而增大,近中根牙根表面吸收陷窝增多,牙周膜局部宽度变窄,新生骨样组织增加。结论:本法成功建立再植牙动物模型,该模型稳定可靠,能完整反映再植牙愈合方式,为进一步研究再植牙愈合机理提供了很好的研究模型。Objective ： To establish an animal model of immediate tooth replantation and make further study of the behaviors of healing and absorption in the replanted tooth. Methods ：The experiment was carried out on thirty 6-week-oid SPF Wistar male rats. The unilateral maxillary first molar of each rat were extracted and replanted immediately, nothing was done on the other side which was the control side. The rats were randomiy separated into six groups and were sacrificed on day 1, day 3, clay 7, day 14, day 21 and day 28 after replantation. After sacrificing the rats,we segregated the maxillaries and scanned the samples with X-ray, so as to analyze the shaded area around the mesial root of each tooth with the Image Pro Plus software. At last, tissue slices were made and stained with HE in order to obtain histological results. Results： Radiographers displayed that the areas of root resorption expanded over time. Histologic feature showed that the number of lacuna on root surface increased with time, the width of the periodontal ligament decreased and the number of new bone like tissue increased. Conclusions：The animal model of rat tooth replantation was successfully established, and it was stable and reliable. This model reflected the healing ways and developmental resorption process of replanted tooth completely. It has been provided a good experimental model for the further research of the molecular mechanism of replanted tooth healing.

关 键 词：再植牙 动物模型 

分 类 号：R782.12[医药卫生—口腔医学]