福寿螺纤维素酶的开发与应用  

Studies on exploitation and application ofcellulase from Ampullarium crossean

作　　者：庄总来[1] 周兴旺[1] 唐志军 陈清西[1] 

机构地区：[1]厦门大学生物系,厦门361005

出　　处：《台湾海峡》2000年第1期6-10,共5页Journal of Oceanography In Taiwan Strait

基　　金：福建省自然科学基金资助项目!(C970 0 5号 )

摘　　要：以福寿螺内脏为材料 ,制备纤维素酶粉。测定各组分的酶活力分别为 :内切葡聚糖酶 2 0 .6 5万U/ g ;( β 葡萄糖苷酶 4.2 4万U/ g ;外切葡聚糖酶 0 .48万U/g ;酶粉的蛋白质含量为 37.6 9%、水分为 7.96 %、灰分为 1 .78%。以花生壳作为纤维素酶作用对象 ,测定酶促反应的最适pH为 5 .2 6 ,最适温度为 5 2℃。在 pH5 .2、40℃下 ,纤维素酶作用于花生壳生成葡萄糖 ,在 2h内葡萄糖的生成量与反应时间成正比关系 ,然后逐渐缓慢 ;随着酶浓度的增大 ,葡萄糖的量也呈直线上升 ,说明从福寿螺分离得到的纤维素酶能有效地水解花生壳转化为可被利用的葡萄糖。The enzyme powder of cellulase had been prepared from Ampullarium crossean viscera. It is made up of three kinds of enzyme, which are Endo 1,4 β D Glucanase, Exo 1,4 β D Glucanase, and β 1,4 D Glucosidase. Their specific activities were determined to be 206.5, 4.8, and 42.4 thousand unit per gram, respectively. The protein content of the enzyme powder was determined to be 37.69%, the water content, 7.96% and the ash content,1.78%. The optimum pH of the enzyme powder for the hydrolysis of peanut shell is 5.26, the optimum temperature is 52℃. At pH5.2, 40℃, the cellulase powder can act on peanut shell and produce glucose, and the product quantity increases with prolonging the reaction time and enlarging the amount of cellulase powder.

关 键 词：福寿螺 纤维素酶 制备与应用 

分 类 号：Q556.2[生物学—生物化学] Q959.212.3