胃癌hMLH1基因启动子CpG岛甲基化  

Hypermethylation of CpG island at the hMLHl gene promoter in gastric carcinomas

作　　者：姬瑞[1] 郭娴[1] 郭庆红[1] 陈兆峰[1] 李强[1] 周永宁[1] 杨挚[1] 

机构地区：[1]兰州大学第一医院消化内科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2012年第2期1-3,9,共4页Journal of Lanzhou University（Medical Sciences）

基　　金：兰州大学中央高校基本科研业务费专项资金(lzujbky-2009.149)

摘　　要：目的采用特异性甲基化聚合酶链式反应方法检测hMLH1基因启动子CpG岛甲基化水平。方法对2株胃癌细胞株31例胃癌组织及23例癌旁组织的hMLH1基因启动子CpG岛甲基化水平进行检测。结果对照组均检测到CpG岛甲基化,胃癌细胞株均检测到CpG岛甲基化,11例胃癌组织检测到CpG岛甲基化,7例癌旁组织检测到CpG岛甲基化。胃癌组织hMLH1基因CpG岛甲基化与分化程度及淋巴结转移之间无相关关系。结论胃癌存在hMLHl基因启动子CpG岛甲基化,并且可能是胃癌发生的早期分子事件。Objective To determine the Hypermethylation of CpG island at the hMLH1 gene promoter by utilizing methylation-specific polymerase chain reaction analysis. Methods Tile methylation stata of the hMLH1 promoter CpG-island were investigated in 2 human gastric cancer cell lines, 31 primary human gastric carcinomas, and 23 adjacent gastric tissues. Results CpG- island hypermethylaion was not detected in control biopsy specimens but identified in human gastric cancer cell lines and 11 of gastric carcinoma patients. CpG-island hypermethylaion of hMLH1 gene was detected in 7 adjacent gastric tissues. In primary gastric tumors, a significant association was not found between tumor differentiation status and lymph node metastasis and CpG island methylation status of hMLH1 gene. Conclusion Hypermethylation of CpG island at the hMLH1 gene promoter is frequently detected in gastric carcinoma, hMLH1 promoter hypermethylation may involve in the early gastric carcinogenesis process.

关 键 词：HMLH1 胃癌 CPG岛 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]