地塞米松、油酸、乳酸对体外培养脂肪细胞HSL mRNA表达的影响  

Effects of dexamethasone,oleic acid and lactic acid on the expression of HSL mRNA in adipocytes cultured in vitro

作　　者：王娅[1] 邓俊良[1] 王哲[2] 左之才[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康重点实验室/环境公害与动物疾病四川省高校重点实验室,四川雅安625014 [2]吉林大学畜牧兽医学院,长春130062

出　　处：《黑龙江畜牧兽医》2012年第7期1-4,179,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(3023062);"长江学者和创新团队发展计划"创新团队项目(IRT0848);四川省教育厅重点资助项目(10ZA048);四川农业大学优秀学士毕业论文培育计划项目

摘　　要：为了研究地塞米松、油酸、乳酸对HSL mRNA表达的影响,试验采用实时荧光定量方法检测脂肪细胞中HSL mRNA的表达量。结果表明:油酸、乳酸抑制脂肪细胞内HSL mRNA表达,抑制作用随着浓度增加而增强;地塞米松促进脂肪细胞内HSL mRNA表达,促进作用存在剂量依赖性。To investigate the effects of dexamethasone,oleic acid and lactic acid on the expression of HSL mRNA in adipocytes,the real-time fluorescent quantitative PCR was used to determine the expression levels of HSL mRNA in adipocytes.The results showed that the expression levels of HSL mRNA were inhibited in adipocytes treated with oleic acid and lactic acid,respectively.The inhibition increased with the increase of oleic acid and lactic acid concentrations,dexamethasone could promote the expression of HSL mRNA in adipocytes,and the effect of promotion was dose-dependent.

关 键 词：地塞米松 油酸 乳酸 脂肪细胞 HSL mRNA 

分 类 号：Q813[生物学—生物工程]