一种复合蛋白粉对林可霉素发酵的影响  

Effects of A Complex Protein Powder on Lincomycin Fermentation

作　　者：林惠敏[1] 李继安[1] 毛全贵[2] 张宏周[2] 陈代杰 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040 [2]河南天方药业股份有限公司,河南驻马店463003 [3]中国医药工业研究总院,上海200040

出　　处：《中国医药工业杂志》2012年第7期549-552,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2010ZX09401403;2009ZX09301-007);国家自然科学基金(81072557);上海市自然科学基金(09ZRl430800)

摘　　要：经前期摇瓶试验筛选获得一种能够提高林可霉素发酵水、平的复合蛋白粉,使摇瓶发酵单位提高至4500μg/ml。在200 L发酵罐放大试验过程中,结合中间补料,考察了氮源对林可霉素产生菌菌丝生长及产物合成的影响。结果表明,含有复合生白粉的培养基中,溶解磷浓度显著高于对照组和其他试验组,显著延缓菌丝自溶,使林可霉素发酵单位提高至9561μg/ml。添加相相同浓度无机磷的培养基试验组虽然同样能够延缓菌丝的自溶和促进林可霉素的合成,但其提高能力远低于含复合蛋白粉培养基组。推测复合蛋白粉中的有机磷源可能在林可霉素的,生物合成过程中起重要作用。A complex protein powder was obtained during medium optimization test in flasks. It could increase the production of lincomycin to 4 500 μg/ml. Further studies were carried out in a 200 L fermenter together with batch feed tests. The results showed that the complex protein powder could significantly delay mycelia autolysis and increase the production of lincomycin to 9 561 μg/ml. The concentration of dissolved phosphorus in the test medium containing the complex powder was higher than that in the control. By adding inorganic phosphorus to the control with the similar concentration of the test medium, the results showed that inorganic phosphorus could delay mycelia autolysis and increase the yield of lincomycin, but the capability was lower than that of the complex protein powder. It was speculated that organic phosphorus in the complex protein powder might play an important role in the fermentation of lincomvcin.

关 键 词：林可霉素 发酵 复合蛋白粉 分批补料 

分 类 号：TQ465.9[化学工程—制药化工]