HPLC法测定眼明熏洗液中阿魏酸含量  

Determination of ferulic acid in Yanmingxunxi lotion by HPLC method

作　　者：付玲[1] 张海英[1] 

机构地区：[1]新疆医科大学附属中医医院药学部,乌鲁木齐830000

出　　处：《新疆医科大学学报》2012年第7期931-933,937,共4页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金项目(200821180)

摘　　要：目的建立眼明熏洗液中阿魏酸的高效液相色谱(HPLC)含量测定方法。方法样品用70%甲醇超声提取,过滤,取续滤液,采用反相高效液相色谱(RP-HPLC)法直接测定。用Symmetry shield C18(3.9mm×150mm,5μm)分析柱,柱温:25℃;流动相:甲醇-1%醋酸溶液(35∶65);流速:1.0mL/min;检测波长:321nm;进样量10μL。结果阿魏酸在7.434～37.170μg/mL范围内线性关系良好,r=0.999 9(n=5),平均回收率为99.91%,RSD=1.19%(n=6)。结论 HPLC法测定眼明熏洗液中阿魏酸的含量,操作简单,重复性好,精密度高。Objective To establish high pressure/performance liquid chromatography （HPLC） method for determination of ferulic acid in Yanmingxunxi lotion. Methods The sample was extracted with 70% meth anol through ultrasonic bath and then filtered. The determination was performed by reversed-phase high- performance liquid chromatography （RP-HPLC） on Symmetry shield C18 column （3. 9 mm ×150mn,5μm）, as mobile phase at flow rate of 1.0 mL/min a mixture of methanol-1% ethanoic acid （35： 65） with column temperature of 25 ℃. The dectection wavelength was setat 321 nm. Results Ferulic acid showed good linearity within the concentration average recovery was 99.91%, with RSD of 1. rang 19% e of 7. 434-37. 170 μg/mL, r = 0. 999 9 （n =5）. The （n =6）. Conclusion HPLC determination of the Yan mingxunxi lotion has the feature of simple operation, good reproducibility and could be utilized for the quality control of Yanmingxunxi lotion. precision. This method

关 键 词：眼明熏洗液 阿魏酸 高效液相色谱法 

分 类 号：R283[医药卫生—中药学]