红花矮牡丹花粉贮藏及生活力研究  

Studies on the Pollen Germination and Storage Characteristics of Paeonia Suffruticosa var.spontanea

作　　者：贾文庆[1] 刘会超[1] 刘露颖[1] 

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003

出　　处：《江西农业大学学报》2012年第3期460-463,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：河南省重点科技攻关项目(112102110021)

摘　　要：以红花矮牡丹花粉为材料,采用离体培养法研究不同温度、培养基组分、贮藏温度及时间对花粉萌发的影响,并探讨花粉形态与萌发之间的关系。结果表明:培养基中添加蔗糖和硼酸对花粉的萌发有促进作用,红花矮牡丹花粉萌发培养基中最适宜的蔗糖和硼酸质量分数分别为:9%、0.004 5%,红花矮牡丹花粉萌发率达62.37%,花粉萌发的最佳温度为22℃。花粉生活力随贮藏时间的延长而下降,不同的贮藏条件对牡丹花粉生活力有显著的影响,红花矮牡丹花粉最佳贮藏方法为.196℃超低温保存,耐贮藏力超过365 d。花粉形态与其萌发之间有相关性。The experiment was conducted with the fresh pollens of dwarf peony by in vitro culture to study the effects of culture medium composition,temperature and different storage methods on pollen germination; and the relationship between pollen morphology and germination was studied,too.The results showed that culture media with different concentrations of sucrose and boric acid had good effects on pollen germination. The germinating percentage of dwarf peony pollen reached the highest after incubated on the media supplemented with 9%sucrose and 0.004 5%boric acid,the germination percentage of dwarf peony pollen reached 62.37%.The optimal temperature was 22℃.Pollen vitality gradually descended with the prolongation of storage time.The best environment to keep dwarf pollen vitality was at -196℃.There is a correlation between pollen morphology and pollen germination.

关 键 词：矮牡丹 花粉形态 萌发 生活力 贮藏 

分 类 号：S685.11[农业科学—观赏园艺]