木香降血糖有效部位及有效成分研究  

Active hypoglycemic fractions and constituents in roots of Aucklandiae lappa

作　　者：金清[1] 白晓华[2] 邓亚飞[1] 李学刚[1] 袁吕江[1] 

机构地区：[1]西南大学药学院,重庆400716 [2]洛阳理工学院,河南洛阳471000

出　　处：《中草药》2012年第7期1371-1375,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的筛选木香中降血糖活性部位及主要成分。方法制备木香乙醇提取物,乙醇提取物醋酸乙酯部位、正丁醇部位和水提取部位;用HepG2细胞消耗葡萄糖模型和四氧嘧啶致糖尿病小鼠模型测定各提取物的降血糖活性;降血糖活性强的醋酸乙酯部位用柱色谱法继续分离、精制,得2个化合物,经NMR等鉴定化合物结构;并对2个化合物进行降血糖活性研究。结果木香醋酸乙酯部位有较强的降血糖作用,从木香醋酸乙酯部位中分离得到木香烃内酯和去氢木香内酯,且两者均可降低四氧嘧啶所致糖尿病小鼠的血糖,并显示很好的抗氧化作用。结论木香烃内酯和去氢木香内酯可能是木香的降血糖活性成分,抑制氧化应激从而减轻胰岛素抵抗可能是其降血糖机制。Objective To screen the active constituents in hypoglycemic fractions in the roots ofAucklandiae lappa. Methods The ethyl acetate fraction and butyl fraction were prepared by isolating the ethanol extracts in the roots ofA. lappa using ethyl acetate and butyl alcohol, and the remaining water phase was named as water extract. The hypoglycemic effects of these fractions were first screened in glucose consume test by HepG2 ceils, and then confirmed by alloxan-induced diabetic mice. The ethyl acetate fraction with high hypoglycemic activity was further purified using column chromatography, from which two compounds were obtained. They were costunolide and dehydrocostus lactone. Their structures were evaluated by NMR and their hypoglycemie activities were studied. Results The ethyl acetate fraction was more hypoglycemic than butyl alcohol fraction and water extract. Costunolide and dehydrocostus lactone purified from ethyl acetate fraction revealed the significant hypoglycemic effect on alloxan-induced diabetic mice and also had anti-oxidative activities. Conclusion Costunolide and dehydrocostus lactone isolated from the roots ofA. lappa exert hypoglycemic activity through the inhibition of oxidative stress, thereby reducing insulin resistance.

关 键 词：木香 木香烃内酯 去氢木香内酯 降血糖 活性成分筛选 抗氧化 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学] R977.15[医药卫生—中医学]