鱼油干预对大鼠“术后疲劳”的影响  

作　　者：王强[1] 杜璐迪[1] 陈必成[2] 周峰[1] 闫竞一[1] 余震[1] 

机构地区：[1]温州医学院附属第一医院普外科,325000 [2]温州医学院外科实验室,325000

出　　处：《中华临床营养杂志》2012年第3期153-157,共5页Chinese Journal of Clinical Nutrition

基　　金：国家自然科学基金（81070702）、浙江省重点扶植学科资助（07-F03）和浙江省医药卫生科技计划（2011ZDA018）

摘　　要：目的探讨围手术期应用鱼油对大鼠“术后疲劳”的影响。方法60只sD大鼠，经术前1周行为学训练，以其结果为依据筛选出表现较差的12只为血清空白对照组，余48只按随机数字表法随机均分为模型组和鱼油干预组。鱼油干预组于术前3d至术后7d腹腔注射鱼油[2ml／（kg·d）]，模型组同时段注射生理盐水[2ml／（kg·d）]，术后1、3、5、7d，Norris水迷宫和悬尾实验行为学评估，检测外周血白介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α和超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH—PX）活力。结果血清指标：鱼油干预组术后1、3dIL-6[（66．22±8．80）、（56．03±1．19）pg／ml]明显低于模型组[（83．30±10．69）、（82．72±24．27）pg／ml]（P=0．034，P=0．038）；术后1、3、5、7d，rNF．d[（104．36±5．02）、（84．49±7．81）、（64．47±2．89）、（39．29±2．52）pg／ml]明显低于模型组[（120．01±14．99）、（119．68±8．84）、（75．29±2．58）、（41．96±1．65）pg／ml]（P=0．014，P=0．003，P：0．000，P=0．004）；术后1、3、5、7dIL-1B[（155．11±9．08）、（79．39±5．86）、（57．26±16．07）、（35．42±1．53）pg／ml]明显低于模型组[（204．87±30．61）、（198．82±54．83）、（152．12±29．06）、（64．35±2．70）pg／ml]（P=0．024，P=0．002，P：0．000，P=0．000）；术后5dSOD（1．08±0．08）μmol／L明显高于模型组（0．71±0．06）μmol／L（P=0．000）；术后5、7dGSH-PX[（31．21±1．30）、（30．78±1．83）μmol／L]明显高于模型组[（25．03±1．74）、（27．57±3．57）p,mol／L]（P=0．000。P=0．036）。行为学：悬尾实验鱼油干预组术后1、3d挣扎阈上面积[（6620±1390）、（7011±1472）mv·s]较模型组[（4739±1040）、（4344±1130）my-s]明显增加（P=0．048，P=0．043）；累积不动时间[（118．42±10．05）、（101．02±8．68Objective To investigate the effect of perioperative use of fish oil on "post-operative fatigue"(POF) of rat.Methods After one week's preoperative behavior training,12 rats presented poor behavior were excluded from 60 healthy adult male SD rats as the normal controls of serum parameters.The remaining 48 rats were randomly divided into model group and fish oil treatment group by random number table.The fish oil treatment group received 10 days' (3 days before surgery and 7 days after surgery) intraperitoneal injection of fish oil [2 ml/( kg · d) ],and the model group with saline.On the 1st,3rd,5th,and 7th post-operative day,rats were assessed by Morris water-maze and tail suspension test.Serum levels of interleukin ( IL)-1β,IL-6,tumor necrosis factor-α (TNF-α),superoxide dismutase (SOD),and glutathione peroxidase (GSH-PX) were measured.Results Serum parameters:on the 1st and 3rd post-operative day,the IL-6 level in the fish oil treatment group [ (66.22 ±8.80),(56.03 ± 1.19) pg/ml] was significantly lower than in model group [ (83.30 ± 10.69),(82.72 ± 24.27) pg/ml ] (P =0.034,P =0.038 ) ; on the 1 st,3rd,5th,and 7th post-operative day,the TNF-α level in the fish oil treatment group [ ( 104.36 ±5.02),(84.49 ±7.81 ),(64.47 ±2.89),(39.29 ±2.52)pg/ml ] was significantly lower than in model group [ ( 120.01 ± 14.99 ),( 119.68 ± 8.84),(75.29 ± 2.58 ),(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004); onthe1st,3rd,5th,and 7th postoperative day,the IL-1β level [(155.11 ±9.08),(79.39±5.86),(57.26±16.07),(35.42±1.53) pg/ml]was significantly lower than model group [ (204.87±30.61), (198.82±54.83), (152.12±29.06),(64.35 ± 2.70) pg/ml ] ( P =0.024,P =0.002,P =0.000,P =0.000) ; on the 5th postoperative day,SOD ( 1.08±0.08) μmol/L was significantly higher than model group (0.71±0.06) μmoL/L (P=0.000) ; on the 5th and 7th postoperative day,GSH-PX [ (31.21 ± 1.30), (30.78 ± 1.83) μmol/L] was significantly higher than model group [ (25.03 ±1.74),(27.57±3.57) μ mol/L] (P=0.000,P=0.036).Behavio

关 键 词：小动物“术后疲劳” 鱼油 炎症 开放式腹部手术 行为学 

分 类 号：R285.5[医药卫生—中药学]