香烟对大鼠肺及A549细胞IL-1,6,8表达影响的实验研究  

Influence of cigarette smoke on the expression of IL-1,6,8 in rats' lungs and A549 cell

作　　者：李文芳[1] 万丹[1] 刘福荣[1] 石梦蝶[1] 

机构地区：[1]武汉科技大学医学院预防医学系,湖北430065

出　　处：《现代预防医学》2012年第13期3336-3338,共3页Modern Preventive Medicine

摘　　要：目的研究香烟烟气对肺部炎性介质IL-1,IL-6,IL-8表达的影响。方法 (1)建立动物长期吸烟模型,用放射免疫法检测肺灌洗液(BALF)中IL-1,IL-6,IL-8的表达水平。(2)制备香烟烟气提取物(CSE)并用它染毒A549细胞,检测细胞上清液中IL-1,IL-6,IL-8的含量。结果动物吸烟各剂量组IL-1表达水平与对照组相比差异无统计学意义(P﹥0.05),低、中、高剂量组IL-6,IL-8水平显著升高,差异有统计学意义(P﹤0.05)。CSE染毒的A549细胞培养上清液中IL-1表达水平与对照组相比差异无统计学意义(P﹥0.05),20%、50%、100%CSE组IL-6的表达水平明显升高,IL-8表达水平下降,差异有统计学意义(P﹤0.05)。结论香烟对肺部炎性介质IL-6,IL-8的表达有影响。OBJECTIVE To investigate the influence of cigarette smoke on the expression of inflammatory mediators in lungs.METHODS The animal model of smoking was established.Each group of rats was given smoking by the respective doses for 12 weeks.IL-1,IL-6 and IL-8 in bronchoalveolar lavage fluid（BALF）were detected by radiation immune method.Human lung adenocarcinoma A549 cells were cultured.Mainstream smoke was collected by using dimethyl sulfoxide（DMSO）as absorbent.A549 cells were incubated with different concentrations of CSE（cigarette smoke extract）.After 4 hours the level of IL-1,IL-6 and IL-8 was measured with radioimmunoassay.RESULTS The expression of IL-1 in A549 cells with different concentrations of CSE had no difference with the control cells（P﹥0.05）.Compared with the control group,the IL-6 expressions of 20%,50%,100% concentrations increased（P﹤0.05）,while the expression of IL-8 was decreased by the CSE（P﹤0.05）.The expression of inflammatory mediators in lung of the smoking rat was influenced：For IL-1 in the smoking rat,different dose of smoking had no difference with the control cells（P﹥0.05）.The levels of IL-6 and IL-8 in BALF increased significantly in high-dose group,middle-dose group and low-dose group respectively compared to that of Control group（P﹤0.05）.CONCLUSION Cigarette smoke could influence the expression of IL-6 and IL-8 in lungs.

关 键 词：香烟烟气提取物(CSE) 大鼠 肺灌洗液(BALF) IL-1 IL-6 IL-8 

分 类 号：R-33[医药卫生]