利用STR和Y染色体二等位基因标记鉴定同胞兄弟关系  

Identification of Sibling Brothers Using STR and Y-biallelic Markers

作　　者：汤美云[1] 黄健[1] 蔡金洪[1] 黄晓松[1] 徐玮[2] 瞿志雄[1] 单飞豹[1] 陆惠玲[3] 

机构地区：[1]湖南省第二人民医院芙蓉司法鉴定中心,湖南长沙410007 [2]湖南省人民医院临床医学研究所,湖南长沙410007 [3]中山大学中山医学院法医物证学教研室,广东广州510089

出　　处：《法医学杂志》2012年第3期190-194,共5页Journal of Forensic Medicine

摘　　要：目的通过检测常染色体以及Y染色体二等位基因标记,探讨Y-STR基因座突变情形下的同胞兄弟鉴定策略。方法对被鉴定的两名男性个体,采用Goldeneye 20A试剂盒和18NC试剂盒检验35个常染色体STR基因座,采用PowerPlex誖Y试剂盒和Yfiler试剂盒检验Y染色体上16个STR基因座,根据ITO法计算全同胞指数,采用片段长度差异等位基因特异性PCR或普通PCR法对20个Y染色体二等位基因标记进行分型检测。结果 35个常染色体STR基因座的累积全同胞指数为4.3149×106,两名被鉴定人16个Y-STR基因座有2个发生突变,20个Y染色体二等位基因标记分型结果均相同,支持两个体为全同胞兄弟关系。结论对于发生Y-STR突变的父系鉴定案例,可补充检验Y染色体SNP和InDel等二等位基因遗传标记。Objective To explore the methods for identification of sibling brothers with Y-STR locus muta- tion by detection of genetic markers on autosome and Y-biallelic. Methods Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR. Results Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149×106 from 35 STRs on autosome. Conclusion In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel .

关 键 词：法医遗传学 同胞 染色体 人 Y STR 突变 二等位基因 

分 类 号：R394[医药卫生—医学遗传学]