一株刺参益生菌的发酵配方及其发酵条件优化  

Optimization of fermentation medium and process for a probiotic strain Bacillus subtilis isolated from sea cucumber Apostichopus japonicas

作　　者：元福杰[1,2] 王印庚[2] 廖梅杰[2] 张正[2] 潘鲁青[1] 张述智 

机构地区：[1]中国海洋大学水产学院,青岛266003 [2]中国水产科学研究院黄海水产研究所,青岛266071 [3]青岛中仁药业有限公司,青岛266329

出　　处：《渔业现代化》2012年第3期40-45,共6页Fishery Modernization

基　　金：科研院所技术开发研究专项(2011EG34219);山东省农业良种工程重大课题"速生;抗病;耐高温刺参良种选育";国家自然科学基金项目(30901120);"十二五"863项目(2012AA10A412);青岛市科技计划项目(11-1-11-hy)

摘　　要：以健康刺参肠道的益生菌——枯草芽孢杆菌(Bacillus subtilis)为研究对象,以豆粕、葡萄糖和氯化钠为基础发酵培养基,通过单因素和正交试验,确定该菌株适用于工业生产的最佳发酵培养基配方;通过单因素试验,确定最佳发酵条件;同时在生物反应器中进行扩大培养试验,得到其最佳种龄和发酵时间。试验确立该菌株的最佳培养基配方为:氯化钠10 g/L,葡萄糖2.5 g/L,豆粕20 g/L;最佳发酵条件为:培养温度28℃,初始pH 7.5,转速170 r/min,菌种接种量5%。以最佳发酵条件和培养基,在10 L生物反应器中进行扩大培养试验,得到最佳种龄为24 h、最佳发酵时间为32 h,菌体细胞浓度最高达到8.5×109cfu/mL,芽孢生成率达到91%。研究结果为刺参微生态制剂的工业化生产提供基础数据,为海水养殖专用微生态制剂的开发提供参考。The culture medium composition and fermentation condition were studied on a strain Bacillus subtilis （ CCTCC M2010316）, which has been isolated from healthy sea cucumber Apostichopus japonicus. By univari- ate tests and orthogonal experiment, the optimal medium composition was as follows： glucose 2.5 g/L, soy- bean meal 20 g/L, sodium chloride l0 g/L, and the best fermentation conditions was temperature at 28 ℃, initial pH value 7.5, shaker revolution 170 r/min, bacterial inoculum 5%. The pilot scale cultivation re- vealed that the best inoculum age and fermentation time was 24 h and 32 h, respectively. The highest bacterial concentration was 8.5 × 109 cfu/mL, spores formation rate reached 91%. This paper would provide basic data for large-scale production of sea cucumber probiotics.

关 键 词：刺参 益生菌 枯草芽孢杆菌 发酵工艺 

分 类 号：S917.1[农业科学—水产科学]