实验性ICH血肿周围组织IL-8、ICAM-1的动态变化  

Dynamic changes of IL-8 and ICAM-1 in brain of intracerebral hemorrhage in rats

作　　者：柯进芳[1] 李政军[1] 赵素琴[1] 杜秦川[2] 张海宇[3] 秦毅[4] 

机构地区：[1]宁夏自治区人民医院急诊科,宁夏银川750021 [2]宁夏银川市第一人民医院神经内科,宁夏银川750001 [3]宁夏自治区人民医院放射科,宁夏银川750021 [4]宁夏医科大学解剖教研室,宁夏银川750004

出　　处：《宁夏医学杂志》2012年第6期483-485,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ08134);宁夏自治区卫生厅重点科研项目(NZ08114)

摘　　要：目的动态观察实验性脑出血(ICH)血肿周围组织白细胞介素8(IL-8)、细胞间黏附分子(ICAM-1)的变化及意义。方法将60只SD大鼠随机分为2组,每组又分为5个时间点。采用肝素化VII型胶原酶脑内注入法制做大鼠ICH模型,应用免疫组化技术监测大鼠ICH后血肿周围组织中IL-8、ICAM-1的动态变化。结果假手术组大鼠针道周围组织各个时间点仅见少量IL-8、ICAM-1表达。模型组各个时间点IL-8、ICAM-1表达均增加,与假手术组比较差异有统计学意义(P<0.05)。结论 ICH后大鼠血肿周围组织IL-8、ICAM-1表达均上调,IL-8,ICAM-1参与了ICH的病理过程。观察IL-8、ICAM-1浓度,对判断病情有一定的帮助。Objective To observe the dynamic changes of IL - 8 and ICAM - 1 in brain of intracerebral hemorrhage in rats. Methods 60 Sprague - Dawley rats were divided into two groups and each group was divided into five time points. Collagenase type Ⅶ with heparinize was infused into the right caudate nucleus to build up rat experimental ICH model. The IL - 8 and ICAM - 1 were exam- ined by immunohistochemistry technique. Results A low level of IL - 8 and ICAM - 1 expression in brain started increased after model- ing in every point in sham operation group. The IL - 8 and ICAM - 1 expression increased after intracerebral hemorrhage in model group. Conclusion IL - 8 and ICAM - 1 may participate pathology process of the brain after ICH. It is helpful for predicting the state of an illness by observing the density of IL - 8 and ICAM - 1.

关 键 词：脑出血 白细偶然性介素8 细胞间黏附分子 

分 类 号：R743.34[医药卫生—神经病学与精神病学]