REV3基因联合抗癌药物对SW-480细胞的影响  

Influence of REV3 Gene Combined with Anticancer Drugs on Proliferation and Apoptosis of the SW-480 Cells

作　　者：黄金娟[1] 隋御[1] 李元杰[1] 金彩霞[1] 张竞文[1] 贾伟[1] 李利坚[1] 周翔[1] 徐方[1] 

机构地区：[1]宁夏医科大学基础医学院医学遗传学与细胞生物学系、生育力保持省部共建教育部重点实验室,银川750004

出　　处：《宁夏医科大学学报》2012年第6期561-564,F0003,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(81060170);教育部"春晖计划"项目(Z2011056;Z2008-1-75015);宁夏医科大学校级科研项目(XM200704)

摘　　要：目的探讨REV3基因低表达联合抗癌药物对人结肠癌细胞(SW-480)增殖和凋亡的影响。方法利用RNAi技术降低REV3基因在SW-480细胞中的表达,以实时荧光定量PCR和免疫细胞化学检测REV3表达量的降低情况,选择低表达效率具有统计学意义的细胞作为实验组细胞,再用抗癌药物作用48h后运用MTT实验和流式细胞仪,对实验组和对照组细胞进行细胞增殖和凋亡变化情况的检测。结果与空白对照组相比,REV3基因低表达或抗癌药物(顺铂或槐定碱)单独作用均能促进SW-480细胞凋亡并抑制其增殖(P<0.01);实验组内,与药物空白组相比,REV3基因低表达的同时再联合一种抗癌药物(顺铂或槐定碱),对SW-480细胞凋亡和增殖的作用较单一因素更加显著;若将三者联合则比以上任一两种联合作用效果更为显著,以上结果均具有统计学意义(P<0.05)。结论 REV3基因低表达、顺铂、槐定碱或这三因素两两联合均可促进结肠癌SW-480细胞的凋亡并抑制其增殖,若三者同时联合作用效果更加显著,提示"基因+药物"联合抑癌具有协同增效的作用。Objective To explore influence of REV3 gene combined with anticancer drugs on proliferation and apoptosis of colon cancer cells. Methods The REV3 expression in SW -480 cells were experimentally sup- pressed by the interference RNA technology （RNAi） and were monitored by real -time RT -PCR and immu- noeytochemistry. Compared with untreated or mock - treated cells, ceils with significant reduction of REV3 ex- pression were taken as treatrment groups. Anticancer drugs were used to treat control and REV3 - depleting cells and the single and combined effects were measured by flow cytometry and MTT 48 h after treatments. Re- suits Compared with qRT - PCR control groups, ablation of REV3 or treatment of antieancer drugs （DDP or Sophoridine） alone promoted significantly SW-480 cell apoptosis and inhibit cell proliferation （P 〈 0.01 ）. REV3 depletion in combination with treatment of either drug significantly enhanced the biological effects （ P 〈 0.05 ）. Furthermore, the combined effects of REV3 depletion and treatment with both drugs were still addi- tive. Conclusion Ablation of REV3 significantly enhances treatments of anticancer drugs DDP and qRT - PCR Sophoridine on colon cells regardless of the drugs being applied individually or jointly. This finding sug- gests synergistic interactions between antieaneer drugs and genetic variations, and offers a guideline for cancer chemotherapy.

关 键 词：REV3基因 RNAI技术 SW-480 qRT—PCR 流式细胞仪 

分 类 号：R735.34[医药卫生—肿瘤]