趋化因子受体CXCR7在人肝癌细胞株中的表达及意义  

Expression and Significance of Chemokine's Receptor CXCR7 in Human Liver Carcinoma Cells

作　　者：金栋[1] 马丽[2] 徐俊[1] 胡志鹏[1] 高峰利[1] 殷利军[1] 赵刚[1] 苟伟[1] 王琦[3] 

机构地区：[1]宁夏医科大学总医院心脑血管疾病医院血管外科/普外科,银川750004 [2]宁夏医科大学总医院心脑血管疾病医院药剂科,银川750004 [3]宁夏医科大学总医院肝胆外科,银川750004

出　　处：《宁夏医科大学学报》2012年第6期588-591,共4页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ10153)

摘　　要：目的探讨趋化因子受体(Chemokine receptor,CXCR7)在不同肝癌细胞株中的相对表达强度及意义。方法采用RT-PCR、Western blot法,检测8株不同转移潜能肝癌细胞中趋化因子受体CXCR7在mRNA及蛋白水平的表达情况。结果 CXCR7在8株肝癌细胞中的表达水平差异存在统计学意义,无论是mRNA水平还是蛋白水平,高转移潜能肝癌细胞株(MHCC97-L、MHCC97-H、HCCLM3)CXCR7的表达明显高于普通转移潜能肝癌细胞株(HepG2、PLC/PRF/5、SMMC-7721、BEL-7402、Hep3B),P<0.01。结论 CXCR7与肝癌的发生、发展及预后有一定的相关性。Objective To investigate the expression and significance of CXCR7 in human liver carcinoma cells. Methods The expression of CXCR7 protein and micro - RNA in hepatocellular carcinoma cell lines （ MHCC97 - L, MHCC97 - H, I-ICCLM3, HepG2, PLC/PRF/5, SMMC - 7721, BEL - 7402, Hep3B ） were de- tected with the application of Western blot analysis and RT - PCR analysis, respectively. Results The ex- pression levels of CXCR7 varied significantly among eight cell lines. Western blotting and RT - PCR showed that high metastatic tumor（ MHCC97 -L, MHCC97 -H, HCCLM3 ） cells expressed higher CXCR7 levels than ordinary metastatic tumor （ HepG2, PLC/PRF/5, SMMC - 7721, BEL - 7402, Hep3B） cells （ P 〈 0.01 ）. Conclusion CXCR7 may play a key role in occurrence, development and prognosis of liver cancer.

关 键 词：趋化因子受体 CXCR7 肝细胞肝癌 

分 类 号：R735.7[医药卫生—肿瘤]