腮腺肿瘤中HPV37种亚型的分布  

Distribution of 37 Kinds of Human Papillomavirus Genotypes in Parotid Gland Tumors

作　　者：王妍[1] 陈晓平[2] 薛晓成[1] 张燚[2] 陈群[2] 张竞飞[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]上海市浦东新区公利医院,上海200135

出　　处：《宁夏医科大学学报》2012年第6期596-599,共4页Journal of Ningxia Medical University

摘　　要：目的探讨腮腺肿瘤(Parotid Gland Tumor)与人乳头状瘤病毒(Human Papillomavirus,HPV)感染及其亚型的相关性。方法采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)对59例腮腺肿瘤石蜡包埋组织标本进行37种HPV亚型检测,并以20例健康志愿者的正常口腔黏膜组织为对照。结果腮腺肿瘤石蜡包埋组织标本HPV总检出率为57.6%(34/59),对照组均为阴性(0/20),两组比较差异有统计学意义(χ2=20.234,P<0.05);腮腺良性、恶性肿瘤组织中HPV阳性率分别为59.6%(31/52)和42.9%(3/7),两组HPV阳性率比较差异无统计学意义(χ2=0.189,P>0.05);腮腺肿瘤组中HPV感染亚型以高危亚型为主(80.7%),其中以HPV16、18、52型为主(61.4%);腮腺肿瘤组中HPV单一亚型、多重亚型均可感染,但以混合亚型感染为主(52.9%)。结论腮腺肿瘤的发生可能与HPV感染有相关性。腮腺肿瘤中HPV高、低危亚型均可感染,以高危亚型HPV16、18、52型感染为主。Objective To investigate the distribution of HPV subtypes in the pariotid gland tumors and to e- valuate the relationship between HPV and pariotid gland tumors. Methods 37 HPV subtypes were detected in paraffin -embedded tissues of 59 cases with parotid gland tumors by flow -through hybridization and gene chip（Hybrimax） technology. 20 cases of normal oral mucosa were used as controls. Results HPV general positive rate of pariotid gland tumors was 57.6% （ 34/59 ）. The rate in control group were all negative （ 0/ 20）. The HPV -positive rates of benign tumor and malignent tumor of pariotid gland were 59.6% （31/52） and 42.9% （3/7）. The high- risk HPV（80.7% ） was the most prevalent type in the group of parotid gland tumors and high -risk HPV -16/18/52 were mainly prevalent types（61.4% ）. Both single -HPV and multi- HPV genotype existed in the HPV -positive samples and most of cases（52.9% ） were infected with multi -HPV genotype. Conclusion Incidence of parotid gland tumors may be related with HPV infection. Both high -risk HPV and low -risk HPV genotypes were identify in parotid gland tumors. High -risk HPV subtype was the main subtype in HPV - positive group and high - risk HPV - 16/18/52 were mainly prevalent types.

关 键 词：腮腺肿瘤 人乳头瘤病毒 基因型 导流杂交基因芯片技术 

分 类 号：R739.8[医药卫生—肿瘤]