BMP-2基因修饰自体BMSCs移植促进兔下颌骨牵张成骨新骨形成的实验研究  

EXPERIMENTAL STUDY ON TRANSPLANTATION OF BMP-2 GENE TRANSFECTED AUTOGENOUS BONE MESENCHYMAL STEM CELLS FOR PROMOTING BONE REGENERATION IN RABBIT MANDIBULAR DISTRACTION OSTEOGENESIS

作　　者：黄旋平[1] 周诺[1] 江献芳[1] 杨媛媛[1] 李华[1] 谢庆条[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,南宁530021

出　　处：《广西医科大学学报》2012年第3期329-332,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30760273;30960421);广西自然科学基金资助项目(No.桂科自0991013Z)

摘　　要：目的:检测骨形态发生蛋白-2(BMP-2)基因mRNA及其蛋白的表达,探讨BMP-2基因修饰自体骨髓间充质干细胞(BMSCs)移植对兔下颌骨牵张成骨新骨形成的促进作用。方法:取新西兰白兔36只随机分为3组,每组12只。建立牵张成骨动物模型,在固定期第2天,实验组于牵张间隙注射200μL的BMP-2基因修饰的自体BMSCs液;对照组注射等量自体BMSCs液;空白组注射等量生理盐水。分别于固定2,6周通过逆转录多聚酶链式反应(RT-PCR)、免疫组化等手段检测BMP-2基因mRNA及其蛋白的表达情况。结果:实验组牵张间隙新生骨组织均可见BMP-2基因mRNA和其蛋白强阳性表达。结论:BMP-2基因修饰的自体BMSCs移植能有效促进兔下颌骨牵张成骨新骨形成。Objective：To examine the expressions of both bone morphogenetic protein-2 （BMP-2） gene mR- NA and related proteins and investigate the promotive effect of transplantation of BMP-2 gene transfected autogenous bone mesenchymal stem cells on bone regeneration in rabbit mandibular distraction osteogene- sis. Methods： Thirty-six New Zealand＇s white rabbits were randomly divided into three groups with twelve in each. All objects were prepared into distraction osteogenesis surgical model on right mandibles. On the 2^nd day of consolidation, experimental, control, and blank groups were injected with the same amount of 200 μL of the solution with BMP-2 gene transfected autogenous bone mesenchymal stem cells, the solution with autogenous bone mesenchymal stem cells, and physiological saline at distraction gap, respectively. The expressions of BMP-2 mRNA and related proteins were examined by RT-PCR and immunohistochem- istry at the ends of the 2nd and 6th week consolidations, respectively. Results： Strongly positive expression of both BMP-2 gene mRNA and related proteins were confirmed on regenerated bone in distraction gap. Conclusion： The transplantation of BMP-2 gene transfected autogenous bone mesenchymal stem cells could effectively promote bone regeneration in rabbit mandibular distraction osteogenesis.

关 键 词：骨形态发生蛋白-2 基因修饰 骨髓间充质干细胞 牵张成骨 表达 

分 类 号：R782[医药卫生—口腔医学]