单唾液酸神经节苷脂对体外循环后大鼠脑组织一氧化氮合成酶活性的影响  

EFFECT OF MONOSIALOTETERAHEXOSYL GANGLIOSIDE 1(GM1) ON NITRIC OXIDE SYNTHASE OF CEREBRAL TISSUE AFTER CARDIOPULMONARY BYPASS IN RATS

作　　者：张素斌[1] 秦科[1,2] 黄国勇[1] 莫宁[1,3] 梁东科[1] 

机构地区：[1]广西医科大学第一附属医院急诊科,南宁530021 [2]中国人民解放军第303医院器官移植中心,广州军区肝、肾移植中心 [3]广西医科大学药学院

出　　处：《广西医科大学学报》2012年第3期349-352,共4页Journal of Guangxi Medical University

基　　金：广西自然科学青年基金资助项目(No.桂科青0832027)

摘　　要：目的:研究体外循环(CPB)后脑组织的一氧化氮合成酶(NOS)活性的变化及单唾液酸神经节苷脂(GM1)干预后对其的影响。方法:选用雄性SD大鼠,经右颈静脉插管引流,尾动脉插管灌注建立CPB(CBP组),转流时间l h,建立CPB动物模型。随机分为CPB模型组、CPB模型+GM1组、输血组和假手术组,每组10只。脑组织匀浆测定结构型一氧化氮合成酶(cNOS)、诱导型一氧化氮合成酶(iNOS)和NO活性。结果:CPB组脑组织中的iNOS活性由正常的(16.47±2.52)pmol/mg.prot-1.min-1增加到(69.84±8.73)pmol/mg.prot-1.min-1(P<0.01),CPB模型+GM1组则增加到(57.86±7.79)pmol/mg.prot-1.min-1(P<0.01),与CPB组比较差异有统计学意义(P<0.05);CPB组脑组织中的cNOS活性由正常的(57.74±8.78)pmol/mg.prot-1.min-1下降到(32.67±6.61)pmol/mg.prot-1.min-1(P<0.01),CPB模型+GM1组则下降到(42.72±7.23)pmol/mg.prot-1(P<0.05),与CPB组比较差异有统计学意义(P<0.05);CPB组脑组织中的NO含量由正常的(258.91±48.17)pmol/mg.prot-1增加到(713.74±98.43)pmol/mg.prot-1(P<0.01),CPB模型+GM1组则增加到(523.74±87.36)pmol/mg.prot-1(P<0.01),与CPB组比较差异有统计学意义(P<0.05)。结论:CPB脑损伤与iNOS产生大量的NO导致的神经毒性作用有关;GM1在一定程度降低了脑中活化的iNOS含量,减少了NO的分泌释放,具有一定的脑保护作用。Objective：To observe the effect of GM1 on nitric oxide synthase （NOS）, including constitutive nitric oxide synthase （cNOS） and induced nitric oxide synthase （iNOS） following cerebral damage after CPB in rats. Methods： SPF-class adult male SD rats were randomly divided into CPB, CPB＋GM1, trans- fusion, and sham-operated groups. After establishing a rat model of cardiopulmonary bypass, GM1 with 20 mg/kg was added to the priming solution. All rats were anesthetized to collect the whole cerebral tissues for detecting the activity of cNOS, iNOS and NO content after 24 hours. Results： Compared with the con- trol group, the level of iNOS and NO increased significantly （ P 〈0. 05）, while the cNOS activity de- creased significantly （ P 〈0.05） in the CPB group. GM1 administration obviously descended the activity of iNOS and NO and increased the cNOS activity. Conclusion： The rise of iNOS activity could correlate close- ly with brain injury after CPB, and GM1 could reduce iNOS activity and NO content. GM1 has the effect of brain protection against the neurotoxicity of NO.

关 键 词：体外循环 动物模型 神经节苷脂 一氧化氮合成酶 

分 类 号：R-33[医药卫生]