非小细胞肺癌患者Th1/Th2型细胞因子的表达及其临床意义  

Expressions of Th1/Th2 Cytokines in the Peripheral Blood of Patients with Non-Small Cell Lung Cancer and Its Clinical Significance

作　　者：郭晶[1] 张韶岩[2] 李文[3] 区颂雷[2] 李昕[2] 马旭晨[2] 

机构地区：[1]首都医科大学附属北京安贞医院检验科,北京100029 [2]首都医科大学附属北京安贞医院胸外科,北京100029 [3]首都医科大学细胞生物学系,北京100069

出　　处：《临床肺科杂志》2012年第8期1449-1451,共3页Journal of Clinical Pulmonary Medicine

基　　金：首都医科大学基础临床科研合作课题(10JL56)

摘　　要：目的研究辅助性T淋巴细胞(helper T lymphocyte,Th)1、2型细胞因子在非小细胞肺癌(non-small cell lung canc-er,NSCLC)患者外周血中的表达。方法采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测40例NSCLC患者手术前后和20例正常对照外周血中Th1型细胞因子白细胞介素-2(interleukin-2,IL-2)、肿瘤坏死因子-α(tumor necrosis fac-tor-α,TNF-α)和Th2型细胞因子IL-10、转化生长因子-β1(transforming growth factor-β1,TGF-β1)的水平。结果 NSCLC组术前IL-2、TNF-α水平较正常对照组明显降低(P<0.05);IL-10、TGF-β1水平则明显升高(P<0.05)。NSCLC患者手术后IL-2、TNF-α水平较手术前明显升高(P<0.01);IL-10、TGF-β1水平则明显降低(P<0.01)。外周血Th1/Th2型细胞因子水平与NSCLC临床病理无关。结论①NSCLC患者呈现Th1抑制和Th2极化状态,是肺癌发生免疫耐受的可能机制;②手术切除肿瘤可在一定程度上逆转Th1/Th2的失衡状态,改善机体免疫状态,对预后产生积极作用。Objective To investigate the changes of helper T lymphocyte （Th） cytokines in the peripheral blood of patients with non-small cell lung cancer （ NSCLC ）, and their relationships with clinical pathology and operation therapy of NSCLC. Methods The ex- pressions of the Th1/Th2 cytokines including Th1 ： interleukin-2 （IL-2） , tumor necrosis factor-α （ TNF-α）, Th2 ： interleukin-10 （IL-10） , transforming growth faetor-β1（ TGF-β1 ） in the serum samples from 40 patients with NSCLC before and after operation and 20 healthy con- trois were detected by enzyme-linked immunosorbent assay （ELISA）. Results The levels of IL-2, TNF-α in NSCLC group were signifi- cantly lower than those in healthy control group（ P 〈0. 05 ）, while the levels of IL-10, TGF-β1 were significantly higher（ P 〈0. 05 ）. The levels of IL-2, TNF-α in NSCLC patients after operation were significantly higher than that in NSCLC patients before operation （ P 〈 0. 01 ）, while the levels of IL-10, TGF-β1 were significantly lower（ P 〈 0. 01 ）. The expressions of the Th1/Th2 cytokines had no correla- tions with clinicopathologic characteristics of NSCLC. Conclusion （1） There is a tendency of Th1 suppression and Th2 polarization in NSCLC patients, which may be a mechanism of immunotolerance with NSCLC;（2） To some extent, surgical removal of tumors can reverse the Th1/Th2 imbalance, improve the immune status, and has a positive effect on the prognosis.

关 键 词：非小细胞肺癌 辅助性T淋巴细胞 细胞因子 免疫耐受 

分 类 号：R734.2[医药卫生—肿瘤]