黄淮山羊H-FABP基因多态性及其与肌内脂肪含量的关联分析  

Polymorphism and Association with IMF Content of H-FABP Gene in Huanghuai Goat

作　　者：王兰萍[1] 耿荣庆[1] 冀德君[2] 常洪[2] 

机构地区：[1]盐城师范学院生命科学与技术学院,江苏盐城224051 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《云南农业大学学报（自然科学版）》2012年第4期522-525,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：江苏省高校自然科学基础研究项目(09KJD230001);国家自然科学基金(31101747)

摘　　要：运用PCR-SSCP技术检测黄淮山羊H-FABP基因部分外显子和内含子区域的多态性,采用最小二乘均数法对其与IMF含量的关系进行分析。在2对扩增引物中只检测到1个多态位点,表现为GG和GC两种基因型。GC基因型个体的IMF含量显著高于GG基因型个体,在背最长肌和腿肌中GC基因型个体的IMF含量显著高于GG基因型(P<0.05);在胸肌中,GG基因型与GC基因型个体之间的IMF含量无显著差异(P>0.05)。据此推测,H-FABP基因该多态位点的GC基因型可能是IMF含量性状的有效标记基因型。The polymorphism of partial exon and intron of H-FABP gene was detected by PCR-SS- CP. There were only one polymorphism site occurring in two primer amplifications, where showed two genotypes GG and GC. The association between the polymorphism and IMF content was ana- lyzed and the results revealed that the IMF content of the longissimus dorsi muscles and leg mus- cles from GC genotype individuals was significantly higher than that of GG genotype imlividuals （P 〈 0.05）. Results also demonstrated no significant difference had been de｜coted i. the IMF content of the breast muscles between GG genotype and GC genotype （ P 〉 O. 05 ）. Our experi- ment suggested that the H-FABP gene could be a useful candidate gene in increasing the 1MF cnn- tent of goats.

关 键 词：黄淮山羊 H-FABP基因 PCR—SSCP 肌内脂肪 

分 类 号：S827[农业科学—畜牧学]