细胞外基质金属蛋白酶诱导因子基因对小鼠结肠癌细胞CT26侵袭的影响  

Extracellular matrix metalloproteinase inducer enhances the invasion of CT26 murine colon adenocarcinoma and the matrix metalloproteinase-2 secretion and activity

作　　者：戴凌[1] 谭宁[2] 田小林[1] 温海滨[3] 黄岚珍[2] 

机构地区：[1]桂林医学院附属医院普通外科,541001 [2]桂林医学院生物科学实验中心,541001 [3]广西壮族自治区河池市人民医院肾内科

出　　处：《中国医药》2012年第7期870-872,共3页China Medicine

基　　金：科技部癌基因及相关基因国家重点实验室开放课题

摘　　要：目的探讨细胞外基质金属蛋白酶诱导因子（EMMPRIN）基因对小鼠结肠癌细胞CT26侵袭的影响。方法构建包含EMMPRIN基因编码框的真核表达载体pCMV-HA2-EMMPRIN，通过脂质体转染CT26细胞，经G418筛选，建立稳定表达EMMPRIN基因的CT26细胞株。对照组为CT26小鼠结肠癌细胞，EMMPRIN组为转染EMMPRIN的小鼠结肠癌细胞。通过体外侵袭实验，分析EMMPRINCT26细胞侵袭力的改变。通过蛋白质印迹、酶联免疫吸附测定及明胶酶谱实验分析过表达EMMPRIN，CT26细胞基质金属蛋白酶2（MMP-2）合成、分泌、激活的变化。结果当EMMPRIN基因在CT26细胞稳定过表达后，侵袭实验的结果为EMMPRIN组（98±5）个细胞，对照组（48±6）个细胞（P〈0．01）。蛋白质印迹、酶联免疫吸附测定及明胶酶谱实验结果证实，EMMPRIN可明显促进CT26细胞MMP-2的分泌及激活，但对CT26细胞MMP-2的合成无影响。结论EMMPRIN基因可明显促进小鼠结肠癌细胞CT26的侵袭，并可明显促进CT26细胞MMP-2的分泌及激活。Objective To investigate whether extracellular matrix metalloproteinase inducer（EMMPRIN） can enhance the metastatic ability of murine colon adenocarcinoma cell（CT26）. Methods EMMPRIN was over-ex- pressed in CT26 cells through transfecting pCMV-HA2-EMMPRIN into the CT26 ceils. Invasion assay was utihzed to analyze the invasion of CT26 cells in vitro after EMMPRIN over-expression. The synthesis of matrix metalloprotein- ase-2 （MMP-2） and the secreted and activated MMP-2 were examined by western blot, Enzyme linked immunosor- bent assay（ELISA） and zymography. Results After EMMPRIN over-expression,invasion assay showed that inva- sive cells were 98±5 in EMMPRIN group and 48±6 in control group （ P 〈 0.01 ）. The secreted and activated MMP-2 was up-regulated in EMMPRIN group cell culture media, but the synthesis of MMP-2 was no different be- tween EMMPRIN group and control group. Conclusions Over-expression of EMMPRIN can enhance the CT26 cell invasion and up-regulate the secreted and activated MMP-2 in CT26 cells. The results suggest that EMMPRIN may be involved in cancer metastasis and play an important role in promotion of cancer metastasis.

关 键 词：细胞外基质金属蛋白酶诱导因子 结肠癌细胞 转移 

分 类 号：R735.34[医药卫生—肿瘤]