正交设计优选薏苡茎多糖的酶提取工艺  

Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L. Stems by Orthogonal Design

作　　者：郭立强[1] 黄礼德[1] 张照平[1] 潘廷啟[1] 文全泰[1] 颜祖弟[1] 黄锁义[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《安徽农业科学》2012年第21期10876-10877,共2页Journal of Anhui Agricultural Sciences

基　　金：广西自然科学基金资助课题(2011GXNSFA018046);广西中医药科技专项资助课题(GZKZ 10-128);广西教育厅课题(201106LX425);广西中医药管理局课题(gzzc1047);广西医学科学实验中心开放基金专项资助项目(KFJJ2011-04);右江民族医学院民族医药协会资助项目(20110715)

摘　　要：[目的]确定薏苡茎多糖的最佳酶提取工艺。[方法]利用纤维素酶从薏苡茎中提取多糖,采用正交试验方法进行条件优选,并用分光光度法对提取物进行多糖的含量测定。[结果]检测波长为490 nm;葡萄糖含量在0.010～0.100 mg/ml范围内与吸光度呈良好的线性关系(R=0.999 8);最佳酶提取工艺为:pH值为5,酶用量为6.0 ml,反应温度为40℃,酶解时间为150 min;在此条件下,薏苡茎多糖的平均提取率为19.62%,RSD为0.21%。[结论]优选出的酶提取工艺合理,经济,可行。[Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L.stems.[Method] The cellulase extraction process of polysaccharides from C.lachrymal-jobi stems was optimized by means of orthogonal test design.And the content of polysaccharides in extracts was determined by spectrophotometry.[Result] The wavelength of the maximum absorption was 490 nm.Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100 mg/ml（R=0.999 8）.The optimal technological conditions for the extraction of polysaccharides were as follows： pH value was 5,amount of enzyme was 6 ml,the reaction temperature was 40 ℃,and enzymatic hydrolysis time was 150 min.Under these conditions,the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%.[Conclusion] The enzyme extracting procedure is reasonable,economical and feasible.

关 键 词：薏苡茎 多糖 纤维素酶 正交设计 

分 类 号：S567[农业科学—中草药栽培]