GC-MS测定姜油中α-蒎烯·桉油精和龙脑的含量  

Determination of α-pinene,Eucalyptol and Borneol in Volatile Oil from Zingiber officinale Roscoe by GC-MS

作　　者：马雯芳[1] 谭建宁[1] 徐东来 严克剑[3] 

机构地区：[1]广西中医药大学药学院,广西南宁530001 [2]广西壮族自治区南宁食品药品检验所,广西南宁530001 [3]广西中医药研究院,广西南宁530022

出　　处：《安徽农业科学》2012年第21期11030-11031,11116,共3页Journal of Anhui Agricultural Sciences

基　　金：广西自然科学基金资助项目(2010GXNSFA013201)

摘　　要：[目的]建立GC-MS同时测定姜油中α-蒎烯、桉油精、龙脑含量的方法。[方法]以GC-MS测定姜油中α-蒎烯、桉油精、龙脑的含量。[结果]α-蒎烯、桉油精、龙脑的浓度分别在20.01～300.15μg/ml(r=0.999 5)、34.74～521.10μg/ml(r=0.999 0)、6.48～97.20μg/ml(r=0.999 5)范围成良好线性关系;平均加样回收率(n=6)分别为101.09(RSD 1.35%)、97.78%(RSD 1.86%)、103.28%(RSD1.57%)。[结论]该方法灵敏、快速、准确,可为姜油的质量控制提供依据。[Objective] To establish a method for determination of α-pinene,eucalyptol and borneol in volatile oil from Zingiber officinale Roscoe by GC-MS.[Method] Contents of α-pinene,eucalyptol and borneol in volatile oil from Zingiber officinale Roscoe were determined by GC-MS.[Result] The linearity of α-pinene,eucalyptol and borneol were shown in the range of 20.01-300.15 μg/ml（r=0.999 5）,34.74-521.10 μg/ml（r=0.999 0） and 6.48-97.20 μg/ml（r=0.999 5）.The average recovery rate of α-pinene,eucalyptol and borneol were 101.09% with RSD of 1.35%,97.78% with RSD of 1.86%,and 103.28% with RSD of 1.57%（n=6）respectively.[Conclusion] The determination method is sensitive,rapid and accurate,it could be used as the determination method for Zingiber officinale Roscoe.

关 键 词：姜油 Α-蒎烯 桉油精 龙脑 GC-MS 含量测定 

分 类 号：S632.5[农业科学—蔬菜学]